عزل بكتيريا الأمعائية الساكازاكية من بعض أنواع التوابل

Abstract EN

Enterobacter sakazakii is considered an opportunistic pathogen that has been associated with severe lethal infections especially in neonates, elderly, and Immunocompromised adults.

E.

sakazakii is a Gram negative, facultative anaerobes rod-shaped bacterium.

It belongs to the family Enterobacteriaceae and genus Enterobacter.

Although we don’t know the natural habitat of this bacteria we find that it exists in high rate in herbs and spices which indicates that plant may be this natural habitat.

Our study focused on spices, and contained 59 specimen (mixed, unmixed), which cultivated on general and selective medium, then we detected its present based on a-glucosidase activity by using ESIA medium that contains 5-bromo-4-chloro-3-indolyl-a,D-glucopyranoside (XaGlc) as a substrate which the bacteria hydrolyses it then producing blue-green colony, then we confirmed our results by cultivated these colonies on Tryptic Soy Agar (TSA), after this we chose the typical yellow one to identify it by using biochemical tests and Polymerase chain reaction PCR.

Our results showed that 50% of the examined specimen contained E.

sakazakii.