عزل بعض أنواع البكتيريا و تشخيص من الروبيان Metapenaeus affinis و التعرف على الأنواع المنتجة للهستامين

Abstract EN

Genera and species of bacteria was isolated and identified from various of Shrimp Metapenaeus affinis , which have been brought from region of FAO in the province of Basrah, All samples were examined for the occurrence of bacteria and were identified according to growth characteristics , morphological, and microscopic examinations in addition to biochemical tests and API Identification Kit .

Thirty fife isolates of bacteria were found in shrimp and as a follow: Klebsiella pneumonia sub.

pneumonia, Klebsiella oxytoca, Morganella morganii sub.

Morganii, Enterobacter aerogenes, Enterobacter cloacae, Cronobacter sakazakii , Enterobacter taylorae, Citrobacter freundii, Citrobacter amalonaticus , Proteus marina, Proteus vulgaris , Proteus mirabilis, Hafnia alvei, Escherichia coli, Serratia marcescens, Serratia fonticola,Vibrio vulnificus, Vibrio harveyi , Vibrio fischeri,Vibrio parahaernolyticus ,Vibrio fluvialis, Vibrio alginolyticus, Pseudomonas aeruginosa, Pseudomonas fluorescens, Staphylococcus saprophyticus, Staphylococcus intermedius, Staphylococcus epidermidis, Bacillus pumilus, Bacillus licheniformis, Bacillus subtilis,Bacillus alvei , Aeromonas veronii , Aeromonas sobria, Aeromonas encheleia and Aeromonas schubertii .

The results of determination of histamine which produced from bacteria by using Trypticase Soy broth-Histidine (TSBH), Histamine decarboxylase broth(H.D.B.) and Histamine decarboxylase agar (H.D.A.) showed that all isolated bacteria were histamine producing with various levels expect Hafnia alvei, Aeromonas schubertii, Aeromonas veronii, Aeromonas encheleia and Aeromonas sobria were not produce histamine.