تأثير 4-D، 2 و BA في استحثاث الكالس و تحفيز الأجنة الخضرية و توليد الأفرغ العرضية من زراعة قطع الأوراق الفتية في نبات الكاردينيا المتقزم Gardenia jasminoides Ellis صنف Radicans

Abstract EN

This study was conducted in Plant Tissue Culture Laboratory, Agriculture Collage, Basrah University, Basrah Governorate, Iraq during the period 1/ Mars/ 2014 to 15/ Mars/ 2015.

The aims of this study can be summarized to effect the different of concentrations of 2,4-D and BA to induced of callus, somatic embryos and regeneration of Adeventions shoots of dwarf gardenia (Gardenia jasminoides Ellis.) cultivar 'Radicans' viaculture of base and middle segament of leaves,The present study has revealed that (a) The abundant primary callus formation (100%) could be achieved only from young leaf segments (basal and middle segments) within 10.3 and 15.3 days in MS medium containing 3 mg/L 2,4-D and 0.2 mg/L BA, and under dark conditions.1) Transferring the primary callus to MS medium enriched with 3mg/L 2,4-D for 8 weeks under light conditions led to induce somatic embryos, that can be identified by their nodular consistency and green color.2) While, shifting the primary callus to MS medium enriched with 3 or 5mg/L BA + 0.2mg/L 2,4-D led to converting the primary callus to compact callus, and at the end of incubation period, small adventitious shoots was generating.

(b) Results also showed that white brownish granular callus initiated under dark conditions on the base of the leaf petioles (basal segment) explants grown on MS medium containing 5 mg/L BA+0.2mg/L NAA, then the callus increased to covered whole explant during the end of incubation period.

However, adventitious shoots was generated from primary callus when shifted to MS medium fortify with 1 or 2 mg/L BA.

Results also revealed that the middle segment of the leaf explants failed to form callus on MS medium containing different concentrations of BA used in this study but they became brown and died.