تأثير البيوتين (B7)‎ في بعض مستويات مضادات الأكسدة (غير الأنزيمية)‎ و المعالجة البيروكسيدية للدهن في أمصال دم و أكباد ذكور الأرانب المحلية المعرضة للإجهاد التأكسدي بفعل فوق أوكسيد الهيدروجين

Abstract EN

The present study aimed to investigate the capacity of biotin in preventing oxidative stress induced by hydrogen peroxide H2O2 at a concentration of 1% given with consumed drinking water to local male rabbits.

40 male rabbits aged 7-8 months, were used in this study and were divided randomly into four groups, with 10 rabbits within group, as follow: the first group was given standard forage and normal water and considered as control, the second group was given standard forage and 1% H2O2 in water, the third group was given standard forage and 200 mg biotin /kg body weight in water, the fourth group was given standard forage, 1% H2O2 and 200 mg biotin /kg body weight in water.

All groups were treated daily for 8 weeks.

The results showed that the treatment with H2O2 caused a significant decrease in the concentrations of each total protein, albumin, and globulin, but significant increase in uric acid concentration in blood serum of the treated rabbits was shown compared with the control group.

The result also showed a significant decrease in Glutathione (GSH) concentration, associated with a significant increase in Malondialdehyde (MDA) concentration in blood serum and liver tissue of the treated rabbits with H2O2 compared with control the group.

The above changes indicate the ability of H2O2 to induce the oxidative stress in male rabbits.

The results also showed that the treated rabbits with a dose of 200 mg biotin/kg evoked a significant increase in the concentrations of each total protein, albumin, and globulin.

A significant decrease in uric acid concentration in blood serum and a significant increase in GSH concentration were showed.

This was associated with a significant decline in MDA concentration in blood serum and liver tissue of the treated rabbits compared with the control group.

These results indicate that biotin has a protective effect against oxidative stress induced by hydrogen peroxide.