Qualitative and quantitative analysis of lycium barbarum L. flavonoids from leaves
Other Title(s)
التحليل النوعي و الكميا لفلافونويدات نبات العوسج من الأوراق
Joint Authors
Muhammad, Zaynab Yasin
al-Samirrai, Khulud Wahib Abbud
Source
Iraqi Journal of Pharmaceutical Sciences
Issue
Vol. 25, Issue 2 (31 Dec. 2016), pp.27-31, 5 p.
Publisher
University of Baghdad College of Pharmacy
Publication Date
2016-12-31
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Abstract EN
There is no phytochemical study about Iraqi wild type Lycium barbarum L.
and its active components.
However ;L.barbarum as a traditional Chinese herb possessing vital biological activities , such as prevention of cancer and age-related macular degeneration, is widely used in Asian countries.
The main active components of this plant have been identified as flavonoids, carotenoids especially zeaxanthin and polysaccharides, all of these components have been reported to be closely associated with the health-enhancing effect.
In this study a method for investigating flavonoids from the leaves of wild type L.
barbarum that are grown naturally in Iraqi deserts and then estimate the total quantity of these flavonoids as Quercetin.
For qualitative assay classical chromatographic methods was applied; preparative thin layer chromatography (PTLC).
The application employed different solvent systems for flavonoids separation.
The best mobile phase for extracting and separation different flavonoids from L.
barbarum leaves was chosen for (PTLC) in corresponding with standards to investigate each separated flavonoid for this methods.
Total Flavonoids from leaves extract were calculated as Quercetin flavonoid represented the quantitative assay in this study.
Results showed that the plant is rich with different flavonoids among them (rutin, quercetin, kaempferol, luteolin) and others.
The technique with best solvent system for flavonoids separation is by preparative TLC chromatography with (Chloroform: Glacial acetic acid: Formic acid) in the ratio of (44:3.5:2.5).
Iraqi wild type Lycium barbarum contain about (11.28 mg /g dried leaves ) total flavonoids as quercetin.
American Psychological Association (APA)
Muhammad, Zaynab Yasin& al-Samirrai, Khulud Wahib Abbud. 2016. Qualitative and quantitative analysis of lycium barbarum L. flavonoids from leaves. Iraqi Journal of Pharmaceutical Sciences،Vol. 25, no. 2, pp.27-31.
https://search.emarefa.net/detail/BIM-753189
Modern Language Association (MLA)
Muhammad, Zaynab Yasin& al-Samirrai, Khulud Wahib Abbud. Qualitative and quantitative analysis of lycium barbarum L. flavonoids from leaves. Iraqi Journal of Pharmaceutical Sciences Vol. 25, no. 2 (2016), pp.27-31.
https://search.emarefa.net/detail/BIM-753189
American Medical Association (AMA)
Muhammad, Zaynab Yasin& al-Samirrai, Khulud Wahib Abbud. Qualitative and quantitative analysis of lycium barbarum L. flavonoids from leaves. Iraqi Journal of Pharmaceutical Sciences. 2016. Vol. 25, no. 2, pp.27-31.
https://search.emarefa.net/detail/BIM-753189
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 31
Record ID
BIM-753189