Qualitative and quantitative analysis of lycium barbarum L. flavonoids from leaves

Other Title(s)

التحليل النوعي و الكميا لفلافونويدات نبات العوسج من الأوراق

Joint Authors

Muhammad, Zaynab Yasin
al-Samirrai, Khulud Wahib Abbud

Source

Iraqi Journal of Pharmaceutical Sciences

Issue

Vol. 25, Issue 2 (31 Dec. 2016), pp.27-31, 5 p.

Publisher

University of Baghdad College of Pharmacy

Publication Date

2016-12-31

Country of Publication

Iraq

No. of Pages

5

Main Subjects

Pharmacology

Abstract EN

There is no phytochemical study about Iraqi wild type Lycium barbarum L.

and its active components.

However ;L.barbarum as a traditional Chinese herb possessing vital biological activities , such as prevention of cancer and age-related macular degeneration, is widely used in Asian countries.

The main active components of this plant have been identified as flavonoids, carotenoids especially zeaxanthin and polysaccharides, all of these components have been reported to be closely associated with the health-enhancing effect.

In this study a method for investigating flavonoids from the leaves of wild type L.

barbarum that are grown naturally in Iraqi deserts and then estimate the total quantity of these flavonoids as Quercetin.

For qualitative assay classical chromatographic methods was applied; preparative thin layer chromatography (PTLC).

The application employed different solvent systems for flavonoids separation.

The best mobile phase for extracting and separation different flavonoids from L.

barbarum leaves was chosen for (PTLC) in corresponding with standards to investigate each separated flavonoid for this methods.

Total Flavonoids from leaves extract were calculated as Quercetin flavonoid represented the quantitative assay in this study.

Results showed that the plant is rich with different flavonoids among them (rutin, quercetin, kaempferol, luteolin) and others.

The technique with best solvent system for flavonoids separation is by preparative TLC chromatography with (Chloroform: Glacial acetic acid: Formic acid) in the ratio of (44:3.5:2.5).

Iraqi wild type Lycium barbarum contain about (11.28 mg /g dried leaves ) total flavonoids as quercetin.

American Psychological Association (APA)

Muhammad, Zaynab Yasin& al-Samirrai, Khulud Wahib Abbud. 2016. Qualitative and quantitative analysis of lycium barbarum L. flavonoids from leaves. Iraqi Journal of Pharmaceutical Sciences،Vol. 25, no. 2, pp.27-31.
https://search.emarefa.net/detail/BIM-753189

Modern Language Association (MLA)

Muhammad, Zaynab Yasin& al-Samirrai, Khulud Wahib Abbud. Qualitative and quantitative analysis of lycium barbarum L. flavonoids from leaves. Iraqi Journal of Pharmaceutical Sciences Vol. 25, no. 2 (2016), pp.27-31.
https://search.emarefa.net/detail/BIM-753189

American Medical Association (AMA)

Muhammad, Zaynab Yasin& al-Samirrai, Khulud Wahib Abbud. Qualitative and quantitative analysis of lycium barbarum L. flavonoids from leaves. Iraqi Journal of Pharmaceutical Sciences. 2016. Vol. 25, no. 2, pp.27-31.
https://search.emarefa.net/detail/BIM-753189

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 31

Record ID

BIM-753189