استخدام الغشاء اللقانقي المشيمي لأجنة بيض الدجاج المخصب كنموذج داخل الجسم الحي لدراسة تأثير الديكساميثازون على صورة الدم

Abstract EN

The aim of the present study was to investigate the possibility to use the hen،s Egg test – chorioallantoic membrane (HET- CAM)test as a novel in vivo model to study the effects of dexamethasone on a differential white blood cell counts.The result revealed that, the blood smeared of chick embryos in age 13- 15 days of incubation had to the all types of mature leukocytes .

Because of the short period of chick embryos were treated with dexamethasone (90 minutes ) only Showed did not significantly changes in leukocyte counts (lymphocyte, Heterophil, Monocyte, Esinophil and Basophil) in treated groups with defferint doses of dexamethasone ( 2 , 4 , 8 , 16 , 32 , 64 , 128 mcg gram of total egg weight, topically).

We conclude for our study that, the (HET- CAM) test can be used as in vivo model to study the effects of dexamethasone on deferential leukocyte counts ,because of the blood smear have all types of leukocyte, also this study manifested this model have low cost accompanied with other laboratory animals.