تأثير الإنتروسين المنتج من بكتريا Enterococcus faecalis على البكتريا المسببة للإسهال عند الأطفال و صغار الأغنام

Abstract EN

A total of 300 samples (100 samples from feases healthy adult persons, adult persons suffer from diarrhea and100 feases healthy child and from feases children suffer from diarrhea and 100 samples from feases young sheep suffer from diarrhea ) , taken from various hospitals in the Baghdad city during the period from January to April 2012.

All isolates were subjected to the cultural, microscopical, biochemical examinations by Vitek 2 for identification up to the species.

The results showed that 40 isolates belonged to Enterococcus faecalis of which, 25 isolates from diarrhea cause and 15 isolates from normal flora , 31 isolates of which 18 isolates belonged to E.coli and 5 isolates belonged to E.coliO157:H7 and 8 isolates belonged to Salmonella spp.

All isolates were subjected to cultural ,microscopical, biochemical examinations and used( API 20 –E) strip.

and sensitivity of 31 isolates was tested against (11-9) Antibiotics.

Results revealed that isolates showed multi resistance to antibiotics, All isolates of E.coli and E.coliO157:H7 ,Salmonella spp.resistant to some antibiotics for negative Bacteria and belonging to the group Enterobacteriaceae.

And Detection of the ability of E.

faecalis local isolates to produce enterocin by testing the inhibitory activity agar and broth in two media MRS,BHI and two method against bacteria cause diarrhea.

And The results showed a variety of the local isolates in their inhibitory effect against bacteria cause diarrhea in children and young sheep by inhibitor zone between (12-20) mm and was MRS liquid and solid the best media from the brain heart infusion .