Immunological detection and PCR technique of capripoxvirus infection in goats, cows and buffaloes

Other Title(s)

الكشف المناعي و تقنية تفاعل سلسله البوليمريز لخمج فايروس جدري الكابري في الماعز و الأبقار و الجاموس

Dissertant

al-Yasiri, Ahmad Nasir Fayyd

Thesis advisor

al-Rudhan, Adnan Musa

Comitee Members

Muhammad, Haytham Q.
Muhsin, Rahman K.
Khudayr, Bassam Y.

University

University of Basrah

Faculty

Veterinary College

Department

Department of Microbiology

University Country

Iraq

Degree

Master

Degree Date

2011

English Abstract

In this study indirect ELISA test was conducted on 580 serum samples collected from 244 goats, 146 cows and 190 buffaloes.

Indirect ELISA was applied on all these samples depending on the optical density (OD) value (3mean +3OD) of sera previously prepared from blood of well know healthy and non-vaccinated goats, cows, buffaloes.

One negativity cut-off was selected for each animal sera as follows, goat (0.056), cow (0.083), and (0.104) in case of buffaloes sera.

there are (45%) of tested animal serum samples were seropositivity.

In concern of animal species, the higher overall seropositivity ratio was observed in sera of cows (50%), goats (40.2%) and buffaloes (47.4%).

According to animals sex; in goats there were identical ratio of seropositivity (40%) and (40.2%) in both male and female respectively, while in cows and buffaloes; females showed higher ratio of seropositivity (57.7 and 50%) respectively.

According to age of animal the higher ratio are (41.1%, 57.3% and 50.3% respectively) was observed in first age group.

a significant differences were observed in the seropositivity concerning to age groups and sex of cow, while no significant differences were observed for goats and buffaloes.

According to the place of studied animals; the higher ratio of seropositivity were found in goats, cows and buffaloes (41.5%, 53.6% and 52.6%) respectively was observed in Thi-Qar province and then in Basrah province (5.9%) in goats, (42.9%) in cow and (42.1%) in buffaloes.

According to animals status; high ratio seropositivity was observed in a lived tested animals (41.5%, 53.6% and 52.6% ) in goats, cows and buffaloes respectively.

Depending on the period of the study, there was significant difference in the rate of seropositivity against Capripoxvirus with an overall rate of (45 %).

The higher rate of seropositivity was observed in the sera which collected in February (53.6%) followed by March (49.1%).

The IC-ELISA test was used to detect the Capripoxvirus antigens pneumonic lung suspension.

The IC-ELISA was performed on (142) lung samples and the over all rate of Capripoxvirus antigen (CPVA) was (28.9 %) and the higher rate of CPVA positivity was observed in the first age groups (39.7%) of goats.

there was a significant difference between the two age group concerning the OD mean ± SD value of IC ELISA.

Depending on sex , there was no significant difference in the CPVA positivity in both sex of IC ELISA .

The CPVA based IC-ELISA positive results were highly significant among the period of the study, the higher rate of antigen positivity was observed in march (48.6 %).

In concern to OD mean ± SD ,the higher value was observed in the lungs of goats that was collected in April (0.104 ± 0.039).

Both antigen and antibody based ELISA showed high rate of positive results in the first age group (39.7 % and 41.1 % respectively), while antibody based ELISA showed higher over all positivity (40.2 %) in Comparision to that of antigen based ELISA (28.9 %) concerning the age and sex goats.

There was significant difference between the two age groups in case of antigen based ELISA No significant difference was observed in case of antibody based ELISA .

the higher OD mean ± SD value was observed in the second age group (0.038 ± 0.106) in case of antigen based ELISA while no difference was observed in these value in case of antibody based ELISA.

On the other hand no significant difference and nearly similar rate of positivity in the results of both ELISA concerning the sex of goats.

The PCR analysis was performed on (40) pneumonic lung .

Main Subjects

Veterinary Medicine

No. of Pages

96

Table of Contents

• APA
• MLA
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Language

English

Data Type

Arab Theses

Record ID

BIM-755793