دراسة تحلل المبيدين Atrazin و 2,4 -D بعزلات بكتيرية مطفرة
Other Title(s)
A study of the degradation of Atrazin and 2,4-D herbicide by mutagenic bacterial isolates
Author
Source
Issue
Vol. 2, Issue 2 (31 Dec. 2012), pp.69-78, 10 p.
Publisher
University of al-Qadisiyah College of Agriculture
Publication Date
2012-12-31
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Topics
Abstract EN
Microbial isolates are isolated from 25 agricultural soil samples contaminated by herbicide.
Their ability of degradation atrazin and 2,4-D herbicides are examined .
Two isolates are chosen according to ability for degradation , then isolated and identified by using numbers of culture media and biochemical tests Results have indicated that the bacteria are Escherichia coli (B6) and Pseudomonas fluorescens (B13) , which have been incubated with a number of herbicides concentrations (5.0 -17.5) mg L-1 from atrazin and (5.0 -20.0) mg L-1 from 2,4-D.
Results have shown the degradation increases with increasing the concentrations (65 ، 60)% for isolate B6 , B13 respectively with 12.5 mg L-1 of atrazin and (73، 61)% of 2,4-D with 15.0 mgL-1 of atrazin and (73،61) % of 2,4-D with 15.0 mgL-1 after five days incubation.Chemical mutagen (Nitrosoguanidine) 500 μg mL-1 used to mutant bacteria cell with time (0.5 , 1.0 , 1.5 and 2.0) hr.
one hour is enough to do that , there fore rasio degradation increases (93 , 89) % Atrazin of (B13C6 , B6C3) respectively in 12.5 mgL-1 and (97 , 93) % , 2,4-D in 15.0 mgL-1 .
Achridin orange and sodium Dodecyl sulphate (SDS) are used to curined bacteria plasmids by 2500 μg mL-1 from (SDS) and 200 μg mL-1 from (Achridin orange).
The results show (B13C6 , B6C3) became curing cells.
American Psychological Association (APA)
كريم عبيد حسن. 2012. دراسة تحلل المبيدين Atrazin و 2,4 -D بعزلات بكتيرية مطفرة. مجلة القادسية للعلوم الزراعية،مج. 2، ع. 2، ص ص. 69-78.
https://search.emarefa.net/detail/BIM-755938
Modern Language Association (MLA)
كريم عبيد حسن. دراسة تحلل المبيدين Atrazin و 2,4 -D بعزلات بكتيرية مطفرة. مجلة القادسية للعلوم الزراعية مج. 2، ع. 2 (2012)، ص ص. 69-78.
https://search.emarefa.net/detail/BIM-755938
American Medical Association (AMA)
كريم عبيد حسن. دراسة تحلل المبيدين Atrazin و 2,4 -D بعزلات بكتيرية مطفرة. مجلة القادسية للعلوم الزراعية. 2012. مج. 2، ع. 2، ص ص. 69-78.
https://search.emarefa.net/detail/BIM-755938
Data Type
Journal Articles
Language
Arabic
Notes
يتضمن مراجع ببليوجرافية : ص. 76-77
Record ID
BIM-755938