عزل الخمائر من مباه أنهار مختلفة في محافظة البصرة و تشخيصها بتقنية جهاز فايتك Vitek 2 compact system

Abstract EN

The present study aimed to isolate and identified a yeasts from water and sediments of 25 rivers in Basrah Governorate, Iraq.

The period of collection started from April 2014 to February 2015.

Potato dextrose agar was used to isolate yeasts from filtered water and diluted sediments.

It was possible to obtain 300 pure isolates of yeasts which were identified to species level by one of the advanced techniques using YST Vitek -2 Kit and Vitek-2 Compact System.

Results revealed the identification of 15 species belonging to 5 genera of yeasts,which were Candida, Cryptococcus, Kloekera, Rhodotorula, Stephanoascus.

The results showed that C.

tropicaliswas the most frequent species being isolated from all of rivers at Basrah Governorate with anumber of colonies representing 37.3% of total colonies.

Other species such as Candida glabrata and Cryptococcus laurentii were also common (96% and 84% of sampling sites).

On the other hand, Candidalipolyticawasof very low frequency in BasrahRivers (0.7%).

AL- Maaqal 1 wasconsidered the richest place in yeasts where the total number of yeasts reached 21colonies, whereas Al- Madinasite produced only 6 colonies of yeasts .