Study of some molecular markers associated with repructive performance of April line, baladi black and new-zeland white rabbits

Abstract EN

-Random amplified polymorphic DNA (RAPD), using 15 arbitrary primers, was used to investigate three rabbit genotypes namely Animal Production Research Institute (APRI) line, Baladi Black breed (BB) and New-Zealand White breed (NZW) to detect molecular genetic markers which could be used to distinguish among the studied genotypes.

Moreover associations between theses markers and the studied genotypes reproductive performance traits (Litter size, living litter size, birth weight and birth litter size) were performed.

DNA banding pattern indicated that APRI line did not give any band reaction with primers OPA2 and OPA7, while the other two genotypes gave two distinct bands at least.

So, these primers could be used as associated markers for litter size, living litter size, and birth litter size traits.

The result of RAPD patterns generated, in total, 104 bands, out of them 62 were polymorphic.

APRI line and (BB) genotypes shared most of the bands.

This support their similar origin, since they have the same common ancestors.

However, the other genotype (NZW) has different pattern than other genotypes.

The phylogenetic tree indicated that both genotypes APRI line and (BB) were highly similar than (NZW) which was highly diverged than other genotypes.

This investigation indicates that RAPD markers could be used to characterize genetically different rabbits and indicate association with some reproductive performance