Detection of microdeletions in the AZF region of the Y-chromosome in azospermic Palestinian males

Other Title(s)

الكشف عن طفرات (AZF)‎ على كروموسوم Y عند الرجال الفلسطينيين العديمي الحيوانات المنوية

Dissertant

Taha, Shadin

Thesis advisor

Rappocciolo, Emilia

Comitee Members

Halilih, Samiyah
Awdah, Shukri

University

Birzeit University

Faculty

Faculty of Pharmacy, Nursing and Health Professions

University Country

Palestine (West Bank)

Degree

Master

Degree Date

2016

English Abstract

Infertility is a global concern that affects approximately 10-15% of couples.

Over 30 million males worldwide are infertile-up to 30% of which are azospermic.

Y chromosome microdeletions are one of the most common genetic causes of non-obstructive azospermia.

The aim of this study was to screen a group of infertile males from the West Bank of Palestine to identify the frequency of Y microdeletions and assess the significance of this testing for intracytoplasmic sperm injection candidates.

DNA extracted from blood samples of 41 azospermic males, 50 normospermic males and 2 females were analyzed by PCR to detect 17 sequence tagged sites (STS) along the long arm of the Y chromosome.

The primers were specific to the Azospermia Factor (AZF) regions, AZFa, AZFb and AZFc, where a missing STS indicated a microdeletion.

The results showed that 4/41 cases (~9.8%) were deleted in seven different AZFb and AZFc locations, whereas no microdeletions were found in any of the fertile control samples.

Results indicated that the frequency in the Palestinian population is similar to that of other world populations and suggests the need for AZF screening in Palestine, in particular for azospermic males undergoing testicular sperm retrieval for assisted reproduction

Main Subjects

Nursing

No. of Pages

46

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-780675