Biodegradation of 3,4-dichlorobenzoic acid by Corynebacterium argentoratense
Other Title(s)
التحطيم الحيوي لمركب 4-3 ثنائي حامض الكلوروبنزويك
Dissertant
al-Majali, Shadiyah Salih Fadl
Thesis advisor
Comitee Members
Batarisah, Mufid Isa
al-Tarawinah, Khalid Ahmad
Atruz, Umar Muhammad Falih
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2010
English Abstract
In this study, the bacterium C.
argentoratense was isolated from the wastewater treatment plant of Petra City which is located in southern Jordan.
Its identification was detected using morphological and biochemical characteristics.
The best concentration caused the higher degradation percentage was the 4 mM concentration.
The maximum degradation level of 4mM 3,4-dCBA was achieved in 20 hours of incubation time.
The effect of four incubation temperatures (25, 30,37, and 42oC) were tested to investigate their effect on the biodegradation of 3,4- dCBA .The experiments on the degradation percentage at different incubation temperature were used by two ways; without glucose and plus glucose.
Bacterial cells grown in glucose-MSM media resulted slightly in higher degradation percentage in contrast with the glucose-free-MSM media.
Irrespective whether the culture media contain glucose or not, the C. argentoratense cells optimally degrade the 3,4-dCBA at a pH of 7.5.
C. argentoratense cells were carbon starved for 24 and 48 hours.
The 3,4- dCBA degradation by carbon-starved C.
argentoratense for 48h was more advantageous than that of 24 h starved and nonstarved cells, respectively. the starved cells requested only 20 h to reach the maximal limit of degradation percentage compared to the control cells that were achieved within longer period.
When our isolate was pre-starved for 24 and 48 h, the degradation ability of 3,4-dCBA had clearly commenced after a shorter acclimation period, become slightly faster, and the endpoint (final degradation percentage) was accomplished in a shorter time, compared with that of the non-starved cells.
It is important to unify the optimum conditions in one experiment to inspect the maximal outcome for biodegradation, cell growth and therefore Cl release.
The adaptation of C. argentoratense cells clearly showed an important factor for improving the degradation ability.
It is clearly shown that the 1,2-dioxygenase activitydependent- ortho cleavage pathway for 3,4—dCBA substrate is the only option.
Main Subjects
No. of Pages
63
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Theoretical background.
Chapter Two : Literature review.
Chapter Three : Design and methology.
Chapter Four : Result and discussion.
References.
American Psychological Association (APA)
al-Majali, Shadiyah Salih Fadl. (2010). Biodegradation of 3,4-dichlorobenzoic acid by Corynebacterium argentoratense. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-786198
Modern Language Association (MLA)
al-Majali, Shadiyah Salih Fadl. Biodegradation of 3,4-dichlorobenzoic acid by Corynebacterium argentoratense. (Master's theses Theses and Dissertations Master). Mutah University. (2010).
https://search.emarefa.net/detail/BIM-786198
American Medical Association (AMA)
al-Majali, Shadiyah Salih Fadl. (2010). Biodegradation of 3,4-dichlorobenzoic acid by Corynebacterium argentoratense. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-786198
Language
English
Data Type
Arab Theses
Record ID
BIM-786198
