Prevention of γ-radiation-induced DNA damage in human lymphocytes using a serine-magnesium sulfate mixture
Other Title(s)
الوقاية من أضرار الإشعاع على الحمض النووي في الخلايا الليمفاوية البشرية باستخدام خليط من كبريتات السيرين و المغنيسيوم
Joint Authors
Changizi, Wahid
Bahrami, Muna
Asfahani, Mahbod
Shattat Bu Shahri, Sayyid Wahid
Source
Sultan Qaboos University Medical Journal
Issue
Vol. 17, Issue 2 (30 May. 2017), pp.162-167, 6 p.
Publisher
Sultan Qaboos University College of Medicine and Health Sciences
Publication Date
2017-05-30
Country of Publication
Oman
No. of Pages
6
Main Subjects
Topics
Abstract EN
Ionising radiation has deleterious effects on human cells.
N-acetylcysteine (NAC) and cysteine, the active metabolite of NAC, are well-known radioprotective agents.
Recently, a serine-magnesium sulfate combination was proposed as an antidote for organophosphate toxicity.
This study aimed to investigate the use of a serine-magnesium sulfate mixture in the prevention of γ-radiation-induced DNA damage in human lymphocytes as compared to NAC and cysteine.
Methods: This study was carried out at the Iran University of Medical Sciences, Tehran, Iran, between April and September 2016.
Citrated blood samples of 7 mL each were taken from 22 healthy subjects.
Each sample was divided into 1 mL aliquots, with the first aliquot acting as the control while the second was exposed to 2 Gy of γ-radiation at a dose rate of 102.7 cGy/minute.
The remaining aliquots were separately incubated with 600 μM concentrations each of serine, magnesium sulfate, serine-magnesium sulfate, NAC and cysteine before being exposed to 2 Gy of γ-radiation.
Lymphocytes were isolated using a separation medium and methyl-thiazole-tetrazolium and comet assays were used to evaluate cell viability and DNA damage, respectively. Results: The serine-magnesium sulfate mixture significantly increased lymphocyte viability and reduced DNA damage in comparison to serine, magnesium sulfate, NAC or cysteine alone (P <0.01 each).
Conclusion: The findings of the present study support the use of a serine-magnesium sulfate mixture as a new, non-toxic, potent and efficient radioprotective agent.
American Psychological Association (APA)
Changizi, Wahid& Bahrami, Muna& Asfahani, Mahbod& Shattat Bu Shahri, Sayyid Wahid. 2017. Prevention of γ-radiation-induced DNA damage in human lymphocytes using a serine-magnesium sulfate mixture. Sultan Qaboos University Medical Journal،Vol. 17, no. 2, pp.162-167.
https://search.emarefa.net/detail/BIM-791811
Modern Language Association (MLA)
Changizi, Wahid…[et al.]. Prevention of γ-radiation-induced DNA damage in human lymphocytes using a serine-magnesium sulfate mixture. Sultan Qaboos University Medical Journal Vol. 17, no. 2 (May. 2017), pp.162-167.
https://search.emarefa.net/detail/BIM-791811
American Medical Association (AMA)
Changizi, Wahid& Bahrami, Muna& Asfahani, Mahbod& Shattat Bu Shahri, Sayyid Wahid. Prevention of γ-radiation-induced DNA damage in human lymphocytes using a serine-magnesium sulfate mixture. Sultan Qaboos University Medical Journal. 2017. Vol. 17, no. 2, pp.162-167.
https://search.emarefa.net/detail/BIM-791811
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 167
Record ID
BIM-791811