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Extraction and isolation of β-sitosterol from Iraqi wild Lycium barbarum by different techniques (probe and bath ultrasound, HPTLC and PHPLC)
Other Title(s)
استخلاص و فصل مركب بيتاستوستيرول من نبات العوسج البري العراقي بواسطة تقنيات مختلفة (مسبارد حوض الأمواج الفوق الصوتية، الفصل اللوني للطبقة الرقيقة ذات الإنجاز العالي و الفصل اللوني السائل ذو الإنجاز العالي)
Joint Authors
Saur, Kawkab Yaqub
Abd al-Jalil, Dhaka Zuhayr
Husayn, Ahmad Abbas
Source
Iraqi Journal of Pharmaceutical Sciences
Issue
Vol. 26, Issue 2 (31 Dec. 2017), pp.75-84, 10 p.
Publisher
University of Baghdad College of Pharmacy
Publication Date
2017-12-31
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Abstract EN
Lycium barbarum (Awsaj) is a plant belong to family Solanaceae serves as a good source of bioactive compounds like phytosterols which have many important biological activity.
Literature survey available so far revealed that there was no studies about Iraqi wild Awsaj phytosterols especially ß-sitosterol, there for the objective of this study was to examine the efficiency of ultrasound assisted extraction (probe and bath) as compared to the conventional (Soxhlet) extraction method for extraction of phytosterols especially β-sitosterol from fruits, leaves, stems and roots of Iraqi wild Awsaj plant.
This goal was achieved by comparing the extraction mass yield, also by a quick and easy approach for identification and quantification of bioactive β-sitosterol in four parts of Awsaj using high performance thin layer chromatography (HPTLC) which confirm the presence of β-sitosterol in four parts of Iraqi Awsaj plant and the results clarify that the root part of Iraqi Awsaj plant extracted by probe UAE give the highest concentration (0.610 mg/ml) of B-sitosterol followed by fruits (0.465mg/ml), leaves(0.405mg/ml) and the least was stems(0.275mg/ml); according to these; isolation and purification of ß-sitosterol from petroleum ether fraction of Awsaj roots was done by preparative HPLC.
The isolated compound (β-sitosterol) was identified by measuring melting point, FT-IR, TLC and preparative HPLC.
American Psychological Association (APA)
Abd al-Jalil, Dhaka Zuhayr& Husayn, Ahmad Abbas& Saur, Kawkab Yaqub. 2017. Extraction and isolation of β-sitosterol from Iraqi wild Lycium barbarum by different techniques (probe and bath ultrasound, HPTLC and PHPLC). Iraqi Journal of Pharmaceutical Sciences،Vol. 26, no. 2, pp.75-84.
https://search.emarefa.net/detail/BIM-796934
Modern Language Association (MLA)
Abd al-Jalil, Dhaka Zuhayr…[et al.]. Extraction and isolation of β-sitosterol from Iraqi wild Lycium barbarum by different techniques (probe and bath ultrasound, HPTLC and PHPLC). Iraqi Journal of Pharmaceutical Sciences Vol. 26, no. 2 (Dec. 2017), pp.75-84.
https://search.emarefa.net/detail/BIM-796934
American Medical Association (AMA)
Abd al-Jalil, Dhaka Zuhayr& Husayn, Ahmad Abbas& Saur, Kawkab Yaqub. Extraction and isolation of β-sitosterol from Iraqi wild Lycium barbarum by different techniques (probe and bath ultrasound, HPTLC and PHPLC). Iraqi Journal of Pharmaceutical Sciences. 2017. Vol. 26, no. 2, pp.75-84.
https://search.emarefa.net/detail/BIM-796934
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 83-84
Record ID
BIM-796934