تحضير لقاح حي مضعف لجدري الأغنام من العزلة المحلية

Abstract EN

The aim of study was preparation of live attenuated vaccine of sheep pox from local isolate.

26 samples (skin scabs) were collected and represented 9 regions Nineveh province, and after 3 serial passages one positive sample was selected for continuous passages until reach to attenuation of the isolate.

The titration of the isolate was done in chicken embryos and sheep skin (flank region).

Two (2) animals were used for challenge test to determine the protection ratio of this isolate.

The results of serial passages showed distinguished red Pock lesions on chorioallantoic membrane, and the attenuation occur at passage 16 with titer (104.49EID50/0.1 ml) in both skin of sheep and chicken embryos.

The protection against challenge virus (103.5 EID50/0.1 ml) was 100%.

The isolate was safe and gave a positive results for the safety test in pregnant ewes and high dose of vaccine and purity.

Thus the isolate can be used for immunization of sheep against sheep pox disease.