Direct detection of Shiga toxin producing by Escherichia coli by Real-Time PCR
Other Title(s)
الكشف المباشر عن سموم الشيكا المنتجة بواسطة الإشريشيا كولاي بتقنية الوقت الحقيقي لتفاعل سلسلة البلمرة
Author
Source
al-Qadisiyah Journal of Veterinary Medicine Sciences
Issue
Vol. 14, Issue 2 (31 Dec. 2015), pp.78-82, 5 p.
Publisher
al-Qadisiyah University College of Veterinary Medicine
Publication Date
2015-12-31
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Abstract EN
Shiga toxin-producing Escherichia coli (STEC) are defined as strains of E.
coli that produce Shiga toxins (stx), which known as important causes of diarrhea in sheep and cattle.
This study was conducted to determine of Shiga toxin 1 producing E.
coli isolates from diarrheal samples of sheep and cattle.
Samples were collected from different fields in Diwanyia city.
A total of 50 diarrhea samples (25 of sheep and 25 of cattle) were subjected to bacterial DNA extraction by using (AccuPrep® Stool DNA Extraction Kit).
The extracted DNA subjected to Real-Time PCR technique for detection of Shiga toxin 1 (stx1) gene.
Results display that sheep are more prevalence to shedding the Shiga toxin 1 producing E.
coli in (5/25) (20%), while in cattle (2/25) (8%) positive samples.
In conclusion the using of Real-Time technique was shown high specific and rapid method in direct detection of (stx1) gene and most the sheep and cattle which infected by diarrhea carried the Shiga toxin 1 producing E.
coli.
American Psychological Association (APA)
Ismail, Jamilah Radi. 2015. Direct detection of Shiga toxin producing by Escherichia coli by Real-Time PCR. al-Qadisiyah Journal of Veterinary Medicine Sciences،Vol. 14, no. 2, pp.78-82.
https://search.emarefa.net/detail/BIM-819251
Modern Language Association (MLA)
Ismail, Jamilah Radi. Direct detection of Shiga toxin producing by Escherichia coli by Real-Time PCR. al-Qadisiyah Journal of Veterinary Medicine Sciences Vol. 14, no. 2 (2015), pp.78-82.
https://search.emarefa.net/detail/BIM-819251
American Medical Association (AMA)
Ismail, Jamilah Radi. Direct detection of Shiga toxin producing by Escherichia coli by Real-Time PCR. al-Qadisiyah Journal of Veterinary Medicine Sciences. 2015. Vol. 14, no. 2, pp.78-82.
https://search.emarefa.net/detail/BIM-819251
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 81-82
Record ID
BIM-819251