دراسة في الزجاج لفعالية بعض الصادات الحيوية في منع التصاق بعض الجراثيم الهوائية المعزولة من لب اللوزتين

Abstract EN

It was proven that biofilm formation had an important role in relapse infections and bacterial resistance against antibiotics in recurrent bacterial infections.

Bacterial biofilm formation in crypts of tonsil's Core is considered as a major factor in the chronicity and treatment failure in patients with recurrent tonsillitis.According to that, we aimed in our study to evaluate the efficacy of some antibiotics recommended in recurrent tonsillitis in preventing the biofilm formation by some bacteria isolated from tonsil's core.

We had worked on five aerobic species, isolated from tonsil's core of children suffering from recurrent tonsillitis and undergoing tonsillectomy, three were Gram Positive Cocci (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes), and two were Gram Negative bacilli (Klebsiella pneumonia, Escherichia coli).

We evaluated efficacy of five different antibiotics, Amoxicillin\ clavulanic acid, Azithromycin, Lincomycin, Cefuroxime, and Ceftriaxone.

Bacterial identification and Disk diffusion test were carried out through standard procedures.

Evaluation of the anti adherence efficacy of the antibiotics were carried out by using "The microtiter plate assay".

Our study shows that Azithromycin has the best efficacy on the Gram Positive Cocci adherence, followed by Cephalosporin, and Augmentin, while Lincomycin increased the adherence 's rate.

Ceftriaxone has the best efficacy on the Gram Negative Bacilli adherence, while Lincomycin and Cefuroxime increased the adherence's rate, and Augmentin and Azithromycin have no effect on the adherence.