تحديد الميتوكسالين في المستحضرات الصيدلانية باستخدام الاستشراب الغازي الشعري
Other Title(s)
Determination of Methoxsalen in pharmaceutical dosage forms using capillary gas chromatography
Joint Authors
صكر، أمير الحاج
مناع، محمد فراس
Source
المجلة العربية للعلوم الصيدلية
Issue
Vol. 4, Issue 9 (30 Sep. 2013), pp.117-124, 8 p.
Publisher
Publication Date
2013-09-30
Country of Publication
Syria
No. of Pages
8
Main Subjects
Abstract EN
An analytical method based on capillary gas chromatography with flame ionization detector (GC–FID) was developed for the direct analysis of Methoxsalen (8-MOP) without derivative reaction, in pure and pharmaceutical dosage forms using Caffeine as internal standard.
The 8-MOP and Caffeine (IS) were dissolved in chloroform and chromatographed on a TRB-5 column (30m×0.25 mm, 0.25 μm), the temperature of GC oven was as follows: initial temperature was 130°C, held for 1 min, increased to 230°C at 20°C/min,and held for 5 min, and then increased to 250°C at 10°C/min with a final hold of 2 min., with nitrogen as carrier gas with flow rate 3 mL/min and FID detection.
The injector port temperature was maintained at 280°C, with split ratio 1:100, and detector temperature was maintained at 300°C.
The method had a chromatographic total run time of 12 min.
The retention times were about 6.8 and 5.7 min for 8-MOP and IS, respectively.
Results of assay and recovery studies were statistically evaluated for its accuracy and precision.
The calibration graph of peak area ratio was rectilinear in the concentration range 10 - 400 μg/mL of 8-MOP, with a relative standard deviation not exceeds 2.12%.
The limit of detection was 1.5μg/mL, and the limit of quantitation was 5 μg/mL.
Correlation coefficient (R2) of the regression equation was greater thane 0.999.
No interference from any components of pharmaceutical dosage forms was observed.
American Psychological Association (APA)
صكر، أمير الحاج ومناع، محمد فراس. 2013. تحديد الميتوكسالين في المستحضرات الصيدلانية باستخدام الاستشراب الغازي الشعري. المجلة العربية للعلوم الصيدلية،مج. 4، ع. 9، ص ص. 117-124.
https://search.emarefa.net/detail/BIM-830037
Modern Language Association (MLA)
صكر، أمير الحاج ومناع، محمد فراس. تحديد الميتوكسالين في المستحضرات الصيدلانية باستخدام الاستشراب الغازي الشعري. المجلة العربية للعلوم الصيدلية مج. 4، ع. 9 (أيلول 2013)، ص ص. 117-124.
https://search.emarefa.net/detail/BIM-830037
American Medical Association (AMA)
صكر، أمير الحاج ومناع، محمد فراس. تحديد الميتوكسالين في المستحضرات الصيدلانية باستخدام الاستشراب الغازي الشعري. المجلة العربية للعلوم الصيدلية. 2013. مج. 4، ع. 9، ص ص. 117-124.
https://search.emarefa.net/detail/BIM-830037
Data Type
Journal Articles
Language
Arabic
Notes
Record ID
BIM-830037