تقييم إضافة تراكيز مختلفة من الغلوتامين و البرولين إلى محلول تمديد محلي التحضير للسائل المنوي عند ذكور الماعز الشامي

Abstract EN

The aim of this study was to evaluate the addition of different concentrations of glutamine and proline to local diluents (Sodium citrate and glucose with egg yolk, EYC) in comparison to Andromed® as a standard diluent in preservation of Shami buck semen.

The experiment was carried out in Ezraa Station- ACSAD during the breeding season in shami goats flock.

Semen was collected from four bucks by artificial vagina.

Motility was evaluated by using microscope and frozen semen was estimated under CASA system.

Results showed that there was a significant effect (P<0.05) of extenders in the rate of motility after dilution, where that Andromed® prevailed over other different extenders (EYC, EYC with 25 mM glutamine, EYC with 50 mM glutamine, EYC with 50 mM proline).

The motility was 74, 59, 58, 54, and 67% respectively.

The motility reduced After freezing to 54, 38, 37, 33, and 42% respectively.

Results of CASA showed significant effect (P<0.05) in MOT from the studied extenders, it was 71, 64, 63, 58, and 65% respectively.

PROG was 57, 50, 47, 41, and 48% respectively.

It wasn,t noticed any significant differences in the studied extenders for other motility indicators.

According to the results of this study these extenders can be used as alternatives of Andromed® for extending shami goat semen as fresh diluted (all undertestable extenders) or frozen (EYC with 50 mM proline).