التقويم المخبري لكفاءة بعض المستنبتات في تخزين النيماتودا الممرضة للحشرات Heterorhabditis bacteriophora

Abstract EN

The efficacy of some substrates for the storage of local isolates of Heterorhabditis bacteriophorawas were evaluated in the Nematology Research Laboratory, Biological Control Research and Studies Center, Faculty of Agriculture, Damascus University during 2016-2017.

Nematodes were isolated from a soil sample collected from Lattakia Governorate and were identified as H.

bacteriophora.

Nematodes were propagated on larvaes of Galleria mellonella.

These individuals were then added to the following substrates: pumice, soil, peat-moss and tourbe.

In addition, pectin, agar, silica gel, and carboxymethyl cellulose (CMC) were added to improve the efficiency of storage substrates.

Each substrate was tested individually, with and without additives.

Survival rates of nematodes were taken at the end of each week for eight weeks.

The average survival rate of nematodes at the end of the storage period was 33.67% for the substrate of the pumice + sand, 32.67% for the pumice + agar and 32.33% for pumice + silica gel, and these were the highest survival rates of living nematodes in this study.

Consequently, these substrates are the most appropriate for laboratory storage of H.

bacteriophora nematodes