علاقة البروتين الورمي p53 مع واسمة التكاثر الخلوي Ki67 و تعبير القرنين الخلوي 17 في السرطان البشروي الفموي شائك الخلايا : دراسة نسيجية مناعية

Abstract EN

Background & Objective: This immunohistochemical study is to investigate the correlation between p53 & Ki67 and the grade of malignancy of squamous cell carcinoma, to estimate functional behavior of the cancerous cell by Ck17, and to compare the results with the histological grading.

Methods & Materials: 64 samples (biopsies) were chosen to check for p53, Ki67 & Ck17 at department of oral histology and pathology between 2002- 2009.

All four degree of malignany were chosen and checked, each degree 16 biopsies.

Samples were chosen of keratinized and non-keratinized mucosa.

Immune reactives Ck17, p53 and Ki67 were used for staining.

Pearson's correlations coefficient was used to study the the correlation between p53, Ki67, Ck17 and the degree of malignancy.

Results: appearance of nucleous 87,5%, polymorphism 65,6%, abnormal mitosis 59,4% and individual keratinization 36% were showen.

Pearson's correlation coefficient means a strong positive correlation between the sings of the cellular atypism and the dgree of malignancy.

P53, Ki67 and Ck17 were positive in 76,6%, 51,6% and 65,6% successively.

The correlation between the positivity of immune reactives and the degree of malignancy was strong and significant.

Conclusion: There is a strong positive correlation between the degree of malignancy and the signs of cellular atypism (morphological changes) and the immunhistochemical positivity specially p53 and Ki67, and there is a strong negative correlation between the positivity of p53, Ki67 and the plasmolymphatic infiltrate.

We must be sure of the correct diagnosis of squamous cell carcinoma by histological and immunohistochemical examination, and attention should be given to use more than one immune reactive.