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Detection and identification of the extended-spectrum β-lactamases (bla SHV and bla CTX-M) Klebsiella pneumonia by PCR technique
Joint Authors
al-Hasnawi, Zaynah Fuad Salih
Husayn, Mushtaq Talib
Khalaf, Siba Falah
Source
al-Qadisiyah Journal of Veterinary Medicine Sciences
Issue
Vol. 16, Issue 2 (31 Dec. 2017), pp.22-28, 7 p.
Publisher
al-Qadisiyah University College of Veterinary Medicine
Publication Date
2017-12-31
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Abstract EN
Klebsiella pneumonia was the most important nosocomial infections pathogen.
It was causing several morbidity and mortality in sick animals and human.
Its identification and detection performed by usage of conventional cultural characters, biochemical tests and Polymerase chain reaction technique (PCR).
One hundred clinical samples were divided into (50) samples of sheep suffering from pneumonia and (50) samples of a human with UTI, collected from different regions of Al-Diwaniyah city.
Thirty-four (68%) sheep samples were positive for K.
pneumonia identification, while 38 (76%) urine samples of a human with UTI cases gave K.
pneumoniae isolates.
The results show only (72) isolates were identified by PCR technique.
Thirty isolates of human samples (78.9%) were positive for detection of bla CTX-M ESBLs, while its detection did not determine in sheep.
Sixty-three isolates from total isolates were positive to detection of bla SHV, these positive isolates divided into 53 isolates for human and 28 sheep.
Molecular characterization of ESBL provides information about the prevalence of ESBL producing K.
pneumoniae in Al-Diwaniyah.
The aim of this study was the determination of CTX-M and SHV genes presence in extended-spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae.
American Psychological Association (APA)
al-Hasnawi, Zaynah Fuad Salih& Husayn, Mushtaq Talib& Khalaf, Siba Falah. 2017. Detection and identification of the extended-spectrum β-lactamases (bla SHV and bla CTX-M) Klebsiella pneumonia by PCR technique. al-Qadisiyah Journal of Veterinary Medicine Sciences،Vol. 16, no. 2, pp.22-28.
https://search.emarefa.net/detail/BIM-847104
Modern Language Association (MLA)
al-Hasnawi, Zaynah Fuad Salih…[et al.]. Detection and identification of the extended-spectrum β-lactamases (bla SHV and bla CTX-M) Klebsiella pneumonia by PCR technique. al-Qadisiyah Journal of Veterinary Medicine Sciences Vol. 16, no. 2 (2017), pp.22-28.
https://search.emarefa.net/detail/BIM-847104
American Medical Association (AMA)
al-Hasnawi, Zaynah Fuad Salih& Husayn, Mushtaq Talib& Khalaf, Siba Falah. Detection and identification of the extended-spectrum β-lactamases (bla SHV and bla CTX-M) Klebsiella pneumonia by PCR technique. al-Qadisiyah Journal of Veterinary Medicine Sciences. 2017. Vol. 16, no. 2, pp.22-28.
https://search.emarefa.net/detail/BIM-847104
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 27-28
Record ID
BIM-847104