Lactate dehydrogenase and β-glucuronidase as salivary biochemical markers of periodontitis among smokers and non-smokers

Abstract EN

Objectives: This study aimed to establish lactate dehydrogenase (LDH) and β-glucuronidase as salivary biomarkers of periodontitis among smokers and non-smokers.

Methods: This cross-sectional case-control study was conducted at the Aligarh Muslim University, Aligarh, India, between January and June 2017.

A total of 200 participants were divided into four groups based on their periodontal and smoking statuses.

Unstimulated mixed saliva samples were collected to estimate LDH and β-glucuronidase levels.

In addition, total protein was estimated using Lowry’s method.

Results: There was a significant increase in enzyme activity in the periodontitis groups compared to the nonperiodontitis groups (P <0.001).

However, significantly lower enzyme activity was observed among smokers, irrespective of periodontal status (P <0.001).

Nevertheless, a receiver operating characteristic curve analysis indicated the diagnostic potential of both enzymes to be fair-to-excellent.

Conclusion: Although smoking was found to significantly alter enzyme activity, LDH and β-glucuronidase were reliable salivary biomarkers of periodontitis among both smokers and nonsmokers.