توصيف إنزيم ال β-Galactosidase المستخلص و المنقى من ضرع البقر
Other Title(s)
Characterization of β-Galactosidase extracted and purified from bovine udder
Joint Authors
إياد نافع يحيى
علي نوري عبد
عزة، هبة عدنان إبراهيم
Source
مجلة جامعة دمشق للعلوم الأساسية
Issue
Vol. 32, Issue 1 (30 Jun. 2016), pp.117-135, 19 p.
Publisher
Publication Date
2016-06-30
Country of Publication
Syria
No. of Pages
19
Main Subjects
Abstract EN
Some characteristics of the enzyme β-galactosidase extracted and purified from the udder of cowswere studiedin the laboratory of Graduate Studies in the Department of Science - College of Basic Education - University of ALMustansiriya.
This study had confirmed that this enzyme is glycoprotein in which the carbohydrate part constitutes (20.5%) according to phenol –sulfate acid method, and its molecular weight was 176.620 KD as estimated by gel filtration.
The total enzymatic effectiveness was(3000) units/ ml at the optimized pH5.5, for enzyme activity, the enzyme had lost its activity completely at pH (9), and showed high stability at the pH (4 - 6.5).
The results showed that the optimum temperature for the highest enzyme activity (1800 Units/ ml )was (55)C° at the optimized pH, The temperature stability of the effectiveness of the enzyme ranged between (30-60)C °.
And the ability of the enzyme to hydrolyze lactose, ranged between (22%) after (30) minutes to (92%) after (270) minutes.
American Psychological Association (APA)
إياد نافع يحيى وعلي نوري عبد وعزة، هبة عدنان إبراهيم. 2016. توصيف إنزيم ال β-Galactosidase المستخلص و المنقى من ضرع البقر. مجلة جامعة دمشق للعلوم الأساسية،مج. 32، ع. 1، ص ص. 117-135.
https://search.emarefa.net/detail/BIM-873925
Modern Language Association (MLA)
إياد نافع يحيى....[و آخرون]. توصيف إنزيم ال β-Galactosidase المستخلص و المنقى من ضرع البقر. مجلة جامعة دمشق للعلوم الأساسية مج. 32، ع. 1 (2016)، ص ص. 117-135.
https://search.emarefa.net/detail/BIM-873925
American Medical Association (AMA)
إياد نافع يحيى وعلي نوري عبد وعزة، هبة عدنان إبراهيم. توصيف إنزيم ال β-Galactosidase المستخلص و المنقى من ضرع البقر. مجلة جامعة دمشق للعلوم الأساسية. 2016. مج. 32، ع. 1، ص ص. 117-135.
https://search.emarefa.net/detail/BIM-873925
Data Type
Journal Articles
Language
Arabic
Notes
يتضمن مراجع ببليوجرافية : ص. 133-135
Record ID
BIM-873925