التشخيص الجزيئي لفيروس موازييك و تقزم الذر Maize dwarf mosaic virus (MDMV)‎ و مقاومته أحيائيا باستخدام المكملات الغذائية و بكتريا Bacillus subtilis على عدة تراكيب وراثية من محصول الذرة الصفراء

Abstract EN

This study aimed at diagnosing maize dwarf mosaic virus (MDMV) for the first time in the governorate of Salah al-din, Iraq in locally infected maize plants using rt-pcr, which amplified a specific viral genomic segment of 237 bp.

the study also evaluated the efficiency of various biological agents, including spirulina platensis, mushroom powder Cordyceps sinensis, and Bacillus subtilis and their combined effect in stimulating resistance against MDMV, and on certain growth traits in different maize genotypes.

the overall integrated treatment of the biological factors above was expressed in an increase of leaf area index of 2.36 cm2, compared to 1.95 cm2 for the untreated control.

the same effect was obtained in relation to chlorophyll content, where the highest mean was obtained following the triple treatment (56.97 spad), when the mean for the control treatment was 49.13 spad.

the triple treatment gave the lowest mean of infection rate, which reached 4.15% following the bs + sp treatment and was the highest (8.61%)in the untreated control.

disease severity and its effect on plant yield and its response to the different treatments and maize genotypes was investigated.

the highest disease severity index of 1.36 was obtained for the treatment with b.

subtilis, and the lowest for the untreated control (0.54).