Characterization and purification of alkaline proteases from viscera of silver carp (hypophthalmichthys molitrix)‎ fish

Abstract EN

Proteases from viscera of silver carp (hypophthalmichthys molitrix) fish have been extracted and characterized.

the alkaline proteases show optimum activity in 0.2m tris-hcl buffer at ph 8.5 and 45°c using soluble milk casein as substrate.

the crude alkaline protease lost about 35% or51% of its specific activity if it was heated at 50c or kept at ph 9 for 60 min., respectively.

purification of proteases purified by ammonium sulfate precipitation, gel-filtration using sephadex g 50 column and ultrafiltration via polyamide membrane (30 kda) led to increase its specific activity up to20, 24 and 99 fold, respectively.