تأثير فصل السنة و عمر الأم المانحة في إنتاج الأجنة خارج الرحم في المعز السوري

Abstract EN

This research was conducted at the laboratories of the National Commission for biotechnology in Damascus during period1/3/2013-28/2/2014 to study the effect of season and donor's age in produce goat embryos in vitro and the rate of survivability of post vitrified- thawed embryos.

Ovaries of slaughtered goats collected throughout the year approximately 10-15 minutes post slaughtering were placed immediately into PBS supplemented with gentamycin at 37 ̊C and transported to the laboratory within 2 hours.

Each ovary was sliced with a sharp tool along the whole surface and washed with warm medium (PBS).

Oocytes were divided according to the year seasons and based on goat's age into three groups (<1 year, 1-2 years, > 2 years).

Morula were vitrified with DMSO 15% + EG 15% into liquid nitrogen.

Data were analyzed according to GLM using SPSS 17, and two ways analysis of variance (ANOVA) were applied to determine the effect of studied factors and F-Test was used to compare the means.

Results indicated that slicing technique allowed to collect 1474 oocytes (20 oocyte/ ovary) with overall rates of maturation, fertilization, cleavage and survivability of post vitrified- thawed embryos were 42.54%, 29.35%, 28.8% 47.17%, respectively.

Analysis of variance showed significant effects (P<0.01) for the season, donor's age and their interactions on studied traits.

It was concluded that oocytes collected during the summer and autumn from donors aged > 1 year had the best competence to produce embryos In Vitro and showed the highest survival rate against vitrification and thawing processes, to produce many of embryos for other uses in research and applied fields.

and it is recommended that more investigations are needed to develop the competence of oocytes obtained from prepubertal donors to enable embryo production ex vivo