الإكثار الخضري الدقيق لنبات المغد الأسود Solanumnigrum L.

Abstract EN

This research aimed to study the effect of some growth regulators on the propogation and the rooting ratio of Solanum nigrumL.

Plantsand to determine the suitable hormone concentration that give the highest multiplication rate and the best rooting.

This study was carried out in laboratory of Biotechnology of Medicinal and Aromatic Plants in National Commission for Biotechnology during 2013-2014.

Seeds were surface disinfected by sodium hypochlorite and planted on free of growth regulators MS medium, in order to secure sufficient plant materials.

After the success of initial culture, plants were transferred to multiplication medium supported with different concentrations of Auxins (NAA or IBA) of 1 mg/l and Cytokinins (BAP or KIN) concentration of 0.5, 1, 1.5, 2 mg/l, and incubated at a temperature of 24±2° Cusing light intensity3000 lux, and then transferred to the rooting media supported with 0.1, 0.5, 1.5, 2 mg/l of IBA rooting hormone.

The rate of rooting was calculated after 40 days from the culture on the rooting medium.

Results showed the absence of pollution when using sodium hypochlorite in the process of disinfection of the surface of the seeds and gave the average germination percentage of 62.22%.

While in multiplication media, control exceeded in plant length parameter in average of10cm and significant differences in comparesion with all other treatments.

While most of used hormonal combinations increased the multiplication rate, where MS.11 and MS.12 media exceeded the rate of multiplication (2.60 branch).

The highest rate of rooting (89.66%) with the highest number of roots (3.33) was observed when using 0.5 mg/l of IBA.

The plants were ed flowering in the most of treatments and it was copious at MS.11 treatment.