تقييم قدرة عزلات محلية من بكتريا المحيط الجذري rhizobacteria للحمص في إنتاج هرمون حمض الإندول الخلي (IAA)‎ Indole-3 acetic acid

Abstract EN

Samples of chickpea plants (cicer arietinum) were collected from six governerates (Damascus countryside, Dara'a, Hama, Tartous, Sweida, and Quneitra) in Syria.

The rhizobacteria were isolated from nodes of these plants, and identified using morphological and some biochemical characters.

Results showed that 15 isolates may belong to genus Rhizobium.

These isolates were evaluated for their ability to produce Indol Acetic Acid (IAA) in the absence and the presence of L-tryptophan (1mg/ml).

Results showed that all isolates can produce IAA in different concentrations in presence of L-tryptphan only; where the isolates R5, R9, R14 produced a high concentration.

These isolates were used to determine the optimal conditions of L-tryptophan concentration and incubation time necessary for the optimum IAA production.

The results also showed at 72h post incubation that the isolates R5,R14 were able to produce the highest level of IAA (42, 104µg/ml) respectively, in presence of 3 mg L-Tryptophan/ml YM medium) while the isolate R9 produced the highest level of IAA 71µg/ml in presence of 2.5mg L-Tryptophan/ml YM medium.