Genotypic and phenotypic diagnosis of Pseudomonas aeruginosa associated with urinary tract infection in diabetic patients and their resistance to certain antibiotics

Abstract EN

In this study, 125 cases of urinary tract infection were collected from patients of both sexes at Al Hussein Educational Hospital and Diabetes and Endocrinology Specialist Center in Dhi Qar Governorate for the period from October 2017 to March 2018.

After isolation and diagnosis, 105 isolates were obtained and the number of P.

aeruginosa was 75 (71.42%).

The isolates were tested to 5 types of antibiotics and 13 isolates for 16S rRNA detection tests.

The isolates showed high resistance to antibiotics, Gentamycin, Tetracycline and Vanomycin, where the resistance was 100% while the resistance was low for Tobramycin 33.3% and was very sensitive to the antibiotic Imipenem, where the sensitivity was (86.6 %).

The results of 16S rRNA polymerase chain reaction products, was show diagnostic gene within the expected size of the 1500 bp, some isolates were recorded as new strains in the NCBI database.