Hypoxia-induced brain cell damage in male albino wistar rat

Abstract EN

The biochemical markers of rat under low oxygen concentration, including brain water level, lactic acid, necrosis and Na+-K+-ATPase, was detected to analyze the hypoxia-induced brain damage, and to analyze the mechanism of brain injury.

Histopathological alteration in brain tissue induced by hypoxia were investigated through hematoxylin and eosin stain (HE).

Hypoxia induced factor-1a (HIF-1a) expression level in the brain was carried out using immunohistochemistry.

Lactic acid level was positively correlated with the level of hypoxia, while concentration-dependent decrease in total Na+-K+-ATPase activity was noted.

Hypoxia induced rathad a significant difference on brain water content compared to controls.

The level of necrosis and lactic acid level was increased, and the decrease of Na+-K+-ATPase activity was observed.

Histopathological examination of brain confirmed that there was neuronal cell death in hippocampal region.

HIF-1a expression increased the hypoxia adaptation capability of the rat through the expressions of genes.

Lactic acid, Na+-K+-ATPase and HIF-1a plays an important role in brain injury.