![](/images/graphics-bg.png)
Detection of 16S rRNA methylases and Co-Resistance with β-lactams among Klebsiella pneumoniae Isolates from Iraqi Patients
Other Title(s)
تشخيص انزيمات مثيلة 16S rRNA و المقاومة المشتركة مع البيتالاكتام في عزلات Klebsiella pneumoniae من المرضى العراقيين
Joint Authors
Mustafa, Mustafa Suhayl
Abd Allah, Rana Mujahid
Source
Issue
Vol. 16, Issue 3 (30 Sep. 2019), pp.580-587, 8 p.
Publisher
University of Baghdad College of Science for Women
Publication Date
2019-09-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Topics
Abstract EN
Out of 150 clinical samples, 50 isolates of Klebsiella pneumoniae were identified according to morphological and biochemical properties.
These isolates were collected from different clinical samples, including 15 (30%) urine, 12 (24%) blood, 9 (18%) sputum, 9 (18%) wound, and 5 (10%) burn.
The minimum inhibitory concentrations (MICs) assay revealed that 25 (50%) of isolates were resistant to gentamicin (≥16μg/ml), 22 (44%) of isolates were resistant to amikacin (≥64 μg/ml), 21 (42%) of isolates were resistant to ertapenem (≥8 μg/ml), 18 (36%) of isolates were resistant to imipenem (4- ≥16μg/ml), 43 (86%) of isolates were resistant to ceftriaxone (4- ≥64 μg/ml), 42 (84%) of isolates were resistant to ceftazidime (16-64 μg/ml), and 40 (80%) of isolates were resistant to cefepime (4- ≥16μg/ml).
Co-Resistance for both β-lactams and aminoglycosides were detected among 25 (50%) of K.
pneumoniae isolates.
The extended spectrum beta-lactamases (ESBLs) were detected among 25 (50%) of K.
pneumoniae isolates.
Screening of 16S rRNA methylases encoding genes revealed that armA was found in 5 (10%) of K.
pneumoniae isolates, whereas rmtB was not found among K.
pneumoniae isolates.
DNA sequencing of armA revealed that the presence of missense mutations in which affected in the translation of protein by substitutions of amino acids, leading to increase the resistance values of MICs for gentamicin and amikacin.
These variants were registered in NCBI at the accession number LC373258.
The phylogenetic tree of armA variants showed a slight deviation of these variants from K.
pneumoniae species.
American Psychological Association (APA)
Mustafa, Mustafa Suhayl& Abd Allah, Rana Mujahid. 2019. Detection of 16S rRNA methylases and Co-Resistance with β-lactams among Klebsiella pneumoniae Isolates from Iraqi Patients. Baghdad Science Journal،Vol. 16, no. 3, pp.580-587.
https://search.emarefa.net/detail/BIM-899591
Modern Language Association (MLA)
Mustafa, Mustafa Suhayl& Abd Allah, Rana Mujahid. Detection of 16S rRNA methylases and Co-Resistance with β-lactams among Klebsiella pneumoniae Isolates from Iraqi Patients. Baghdad Science Journal Vol. 16, no. 3 (2019), pp.580-587.
https://search.emarefa.net/detail/BIM-899591
American Medical Association (AMA)
Mustafa, Mustafa Suhayl& Abd Allah, Rana Mujahid. Detection of 16S rRNA methylases and Co-Resistance with β-lactams among Klebsiella pneumoniae Isolates from Iraqi Patients. Baghdad Science Journal. 2019. Vol. 16, no. 3, pp.580-587.
https://search.emarefa.net/detail/BIM-899591
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 585-587
Record ID
BIM-899591