Cryopreservation : a tool to conserve date palm in Saudi Arabia
Joint Authors
al-Ansi, Salih
Tarroum, Muhammad
al-Shammari, Arif
Jafar, Abd al-Rahman Z.
Nadim, Muhammad
Khan, Salim
al-Quraini, Fahd
Source
Saudi Journal of Biological Sciences
Issue
Vol. 26, Issue 7 (30 Nov. 2019), pp.1896-1902, 7 p.
Publisher
Publication Date
2019-11-30
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Topics
Abstract EN
The cryostoring of embryogenic tissue of the date palm (Phoenix dactylifera L.
cv.
Sagai) was examined through dehydrated-encapsulation, vitrification, and vitrification-encapsulation.
The most extreme regeneration rate (53.33%) of epitomized, cryostored liquid nitrogen (+LN) treated embryos was observed when pre-embryonic masses were hatched with 0.5 M sucrose for 48 h pursued by 6 h air drying out.
The most noteworthy survival rate (80.0%) of epitomized, cryopreserved embryonic cluster came about when calli were hatched with 0.3 or 0.7 M sucrose for 48 h pursued by four hours of lack of hydration, or with 0.5 M sucrose for 48 h without air drying out or with 2 h of air drying out.
Following cryopreservation utilizing the embodiment vitrification convention, the most astounding survival (86.7%) as well as the greatest growth (46.7%) was accomplished when the typified vitrified, cryopreserved calli were treated with Vitrification Solution 2 for plants (PVS2) for 60 min at 25 °C.
Cryopreservation utilizing the vitrification convention brought about the most extreme recuperation of 53.3%, when vitrified-cryopreserved calli were subjected to PVS2 solution for 30 min at 25 °C.
Most extreme (40%) regeneration of vitrified, cryopreserved embryonic calli was seen when these calli were treated with PVS2 solution for 60 min at 25 °C.
The outcome got amid this investigation of regrowth after cryopreservation of the cv.
Sagai was over the base suitable for a cryo-germplasm bank.
Recovery and regrowth were above 30% for all the techniques developed for the cv.
Sagai.
American Psychological Association (APA)
al-Ansi, Salih& al-Quraini, Fahd& Nadim, Muhammad& Khan, Salim& Tarroum, Muhammad& al-Shammari, Arif…[et al.]. 2019. Cryopreservation : a tool to conserve date palm in Saudi Arabia. Saudi Journal of Biological Sciences،Vol. 26, no. 7, pp.1896-1902.
https://search.emarefa.net/detail/BIM-910438
Modern Language Association (MLA)
al-Ansi, Salih…[et al.]. Cryopreservation : a tool to conserve date palm in Saudi Arabia. Saudi Journal of Biological Sciences Vol. 26, no. 7 (Nov. 2019), pp.1896-1902.
https://search.emarefa.net/detail/BIM-910438
American Medical Association (AMA)
al-Ansi, Salih& al-Quraini, Fahd& Nadim, Muhammad& Khan, Salim& Tarroum, Muhammad& al-Shammari, Arif…[et al.]. Cryopreservation : a tool to conserve date palm in Saudi Arabia. Saudi Journal of Biological Sciences. 2019. Vol. 26, no. 7, pp.1896-1902.
https://search.emarefa.net/detail/BIM-910438
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1901-1902
Record ID
BIM-910438