Ovarian immunohistochemical expression of estradiol 17β in cyclic female rats treated with steroid free bovine follicular fluid antiserum
Joint Authors
al-Jayyashi, G. S. M.
Khafaji, S. S.
al-Saidi, Jabbar Abbas Ahmad
Source
al-Qadisiyah Journal of Veterinary Medicine Sciences
Publisher
al-Qadisiyah University College of Veterinary Medicine
Publication Date
2018-12-31
Country of Publication
Iraq
No. of Pages
8
Main Subjects
English Abstract
The current study aimed to examine the effect of steroid-free bovine follicular fluid (SFBFF) antiserum on ovarian immunohistochemical expression levels of 17β Estradiol in cyclic virgin female rats.
After estrus synchronization, 80 adult virgin female Wistar rats (aged 60 days and weighed 156±4.82 g) were randomly assigned into two equal groups (40 females each) and injected intrperitoneally, at late metestrous, with 100 µl of normal saline (control) and 100 µl of SFBFF antiserum (AI-SFBFF group).
At each phase of the estrus cycle, 10 females from each group were anesthetized (by injection of 0.4 ml of thiopental sod./ animal), dissected and the ovaries were obtained for determination of immunohistochemical expression density of 17β Estradiol.
Ovarian examination of AI-SFBFF group females demonstrated slight increase of estradiol immunohistochemical density at proestrus and mark increase at estrus and metestrus phases.
It could be concluded that immunoneutralization of endogenous inhibin by SFBFF antiserum results in high levels of estradiol actions in reproductive organs.
Data Type
Conference Papers
Record ID
BIM-913034
American Psychological Association (APA)
al-Saidi, Jabbar Abbas Ahmad& al-Jayyashi, G. S. M.& Khafaji, S. S.. 2018-12-31. Ovarian immunohistochemical expression of estradiol 17β in cyclic female rats treated with steroid free bovine follicular fluid antiserum. Scientific Conference : Depending on Scientific Research Approach to develop animal industry and overcome its Problems (6th : 2017 : al-Qadisiyah University, Iraq). . Vol. 17, no. 2 (2018), pp.35-42.a-Qadisiyah Iraq : al-Qadisiyah University College of Veterinary Medicine.
https://search.emarefa.net/detail/BIM-913034
Modern Language Association (MLA)
al-Saidi, Jabbar Abbas Ahmad…[et al.]. Ovarian immunohistochemical expression of estradiol 17β in cyclic female rats treated with steroid free bovine follicular fluid antiserum. . a-Qadisiyah Iraq : al-Qadisiyah University College of Veterinary Medicine. 2018-12-31.
https://search.emarefa.net/detail/BIM-913034
American Medical Association (AMA)
al-Saidi, Jabbar Abbas Ahmad& al-Jayyashi, G. S. M.& Khafaji, S. S.. Ovarian immunohistochemical expression of estradiol 17β in cyclic female rats treated with steroid free bovine follicular fluid antiserum. . Scientific Conference : Depending on Scientific Research Approach to develop animal industry and overcome its Problems (6th : 2017 : al-Qadisiyah University, Iraq).
https://search.emarefa.net/detail/BIM-913034