Genetic diversity of begomoviruses infecting tomato plant in Saudi Arabia

Author

Sohrab, Sayyid Sartaj

Source

Saudi Journal of Biological Sciences

Issue

Vol. 27, Issue 1 (31 Jan. 2020), pp.222-228, 7 p.

Publisher

Saudi Biological Society

Publication Date

2020-01-31

Country of Publication

Saudi Arabia

No. of Pages

7

Main Subjects

Biology

Topics

Abstract EN

Tomato is known as a highly valuable crop and grown worldwide for various uses.

The cultivation and tomato production severely affected globally by several diseases caused by various pathogens.

Begomoviruses causes yellow mosaic and leaf curl disease of tomato in the tropical, subtropical, temperate, and semi-arid regions.

In Saudi Arabia, the tomato production adversely affected by disease caused by begomoviruses known as TYLCV and ToLCSDV.

In this study, the pathogen was identified by Polymerase Chain Reaction using virus-specific primers and transmitted by whiteflies to healthy tomato seedlings.

In a field survey, the tomato plants were exhibiting symptoms like viral infection.

The infected leaf was randomly collected from various fields of tomato growing areas like Jeddah, Makkah, Tabuk, and Hail.

The full-length viral genome was amplified by Rolling Circle Amplification technology (RCA) while betasatellites were amplified by PCR using universal betasatellites primers.

The full-length viral genome (∼2.7 kb) and betasatellites (∼1.4 kb) were cloned and sequenced bi-directionally.

The generated sequences were assembled and analyzed to find out the genetic variability by using bioinformatics tools and the genetic variability and phylogenetic relationships with selected begomoviruses were analyzed.

The sequences showed the highest identity with an isolate of ToLCSDV and TYLCV.

The nucleotide similarity and phylogenetic relationship showed the closest cluster with ToLCSDV and TYLCV.

The data generated in this study elucidate that the causal organism is a variant of either TYLCV or ToLCSDV.

The provided information from this study will be highly valuable for researchers and vegetable growers not only in Saudi Arabia but also in Arabian Peninsula.

American Psychological Association (APA)

Sohrab, Sayyid Sartaj. 2020. Genetic diversity of begomoviruses infecting tomato plant in Saudi Arabia. Saudi Journal of Biological Sciences،Vol. 27, no. 1, pp.222-228.
https://search.emarefa.net/detail/BIM-920572

Modern Language Association (MLA)

Sohrab, Sayyid Sartaj. Genetic diversity of begomoviruses infecting tomato plant in Saudi Arabia. Saudi Journal of Biological Sciences Vol. 27, no. 1 (Jan. 2020), pp.222-228.
https://search.emarefa.net/detail/BIM-920572

American Medical Association (AMA)

Sohrab, Sayyid Sartaj. Genetic diversity of begomoviruses infecting tomato plant in Saudi Arabia. Saudi Journal of Biological Sciences. 2020. Vol. 27, no. 1, pp.222-228.
https://search.emarefa.net/detail/BIM-920572

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 227-228

Record ID

BIM-920572