Effects of 4-CPPU on in vitro multiplication and sustainable generation of Hibiscus rosa-sinensis L. "White Butterfly”

Joint Authors

al-Attar, Abd al-Rahman A.
Abd al-Salam, Islam M.
Qahtan, Ahmad A.
Faysal, Muhammad

Source

Saudi Journal of Biological Sciences

Issue

Vol. 27, Issue 1 (31 Jan. 2020), pp.412-416, 5 p.

Publisher

Saudi Biological Society

Publication Date

2020-01-31

Country of Publication

Saudi Arabia

No. of Pages

5

Main Subjects

Biology

Topics

Abstract EN

There are more than nine thousand cultivars of Hibiscus rosa-sinensis L., with a series of flowers with shapes, colors and new cultivars continues as generated through both traditional and modern breeding techniques.

In this study, advanced biotech methods of in vitro culture have been used to identify a technique for the efficient mass multiplication of H.

rosa-sinensis ‘White Butterfly’, using phenyl urea, N-(2-Chloro-4-pyridyl)-N′-phenylurea (4-CPPU).

For the first time, the effects of 4-CPPU for stimulating axillary shoot proliferation and multiple shoot regenerations from nodal explants were evaluated, and the optimal nutrient media deduced.

From the diverse concentrations as 0.1, 0.5, 2.5, 5.0 & 10.0 µM of 4-CPPU, the highest frequency of shoots was recorded at 2.5 µM supplied in Murashige and Skoog (MS, pH-5.8) medium.

After eight-weeks of culture, on an average of 6.7 shoot were obtained on this media with shoot heights of 4.2 cm from each explant.

With the involvement of 0.5 µM-IBA (indole-3-butyric acid) in MS medium the regenerated shoots were rooted and followed by successful acclimation to ex vitro conditions.

The ploidy consistency among the micro-plants was analyzed using flow cytometry and compared with ex vitro grown plants.

No differences in the ploidy levels were observed among the 4-CPPU induced plants, when compared with the donor plants.

American Psychological Association (APA)

Faysal, Muhammad& al-Attar, Abd al-Rahman A.& Abd al-Salam, Islam M.& Qahtan, Ahmad A.. 2020. Effects of 4-CPPU on in vitro multiplication and sustainable generation of Hibiscus rosa-sinensis L. "White Butterfly”. Saudi Journal of Biological Sciences،Vol. 27, no. 1, pp.412-416.
https://search.emarefa.net/detail/BIM-920704

Modern Language Association (MLA)

Faysal, Muhammad…[et al.]. Effects of 4-CPPU on in vitro multiplication and sustainable generation of Hibiscus rosa-sinensis L. "White Butterfly”. Saudi Journal of Biological Sciences Vol. 27, no. 1 (Jan. 2020), pp.412-416.
https://search.emarefa.net/detail/BIM-920704

American Medical Association (AMA)

Faysal, Muhammad& al-Attar, Abd al-Rahman A.& Abd al-Salam, Islam M.& Qahtan, Ahmad A.. Effects of 4-CPPU on in vitro multiplication and sustainable generation of Hibiscus rosa-sinensis L. "White Butterfly”. Saudi Journal of Biological Sciences. 2020. Vol. 27, no. 1, pp.412-416.
https://search.emarefa.net/detail/BIM-920704

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 415-416

Record ID

BIM-920704