Effect of propolis in diet supplementation on the histopathological changes in some organs and challenge tests against Newcastle disease in broiler chicks

Abstract EN

The present study was conducted to evaluate the effect of dietary supplementation with propolis on histopathological changes of some tissue and challenge tests against Newcastle disease virus at 21 and 42 day old chicks .

One hundred and eighty Ross broiler chicks were used in day - old they were divided randomly to six groups (30 birds/group), feed and drinking water offered ad libitum and experimental groups were treated as follows : The first four groups received (0.5 , 1 , 2 ,3 ) g propolis/kg in feed respectively, the five group served as positive control received basal diet without propolis but vaccinated and group six served as negative control received basal diet without propolis and non vaccinated.

Histopathological changes in bursa of Fabricius showed normal histology with mild condense proliferation of lymphocyte in the cortex at the G4 as compared with other groups which did not show any pathological lesions.

Histopathological changes in the liver of G 4 showed mono nuclear cell aggregation in liver pranchyma and promenance of kupffer's cells as compared with other groups (G1,G2,G3,G5 and G6)which showed normal tissue.

The microscopical examination of the spleen , kidney and thymus showed no histopathological alteration in all the groups.The challenge test showed that the total mortality was zero in group 4 and 3 and low in groups 1 and 2 as compared with groups 5 and 6.