Nanoencapsulation of insulin by alginate-chitosane matrix by ionotropic pre-gelation technique

Abstract EN

Insulin is the most effective hypoglycemic agent used to treat the diabetes.

Oral administration of insulin is the most comfortable and least restrictive way.

However, it encounters the low absorption of insulin by the digestive tract and its degradation by proteolytic enzymes.

Several strategies have been developed to insulin protection against enzymatic hydrolysis in the gastro intestinal environment.

They also aim to promote absorption and transport of insulin through the intestinal epit helium.

In his context, we encapsulated insulin in alginate/chitosan based nanoparticles by the ionotropic gelation method.

This technique comprises two steps, which are the cross link of alginate with calcium chloride followed by complexation with chitosan.

The nanoparticles obtained are then coated with polyethylene glycol 400.

Particle size and zeta potential were characterized, and encapsulated insulin amount was assessed.