دراسة تشخيصية مقارنة لجراثيم الكليبسيال باستخدام نظام ال Api20E و جهاز ال vitek2 و جهاز ال PCR

Abstract EN

The current Study included 273 samples colected from models of different clinical Samples (urine , Stool, blood , sputum, and swabs from the vagina ,wounds, ear and throat) which have been get on 30 (10.98% ) isolates of Klebsiella from total of various clinical samples.

The rate of Klebsiella from urine samples was 26.6% of the while total 30 samples and The isolation rate from stool and blood sampls was 16.6%, Klebsiella isolated from vaginal swabs with .in wound infection the rat was (13.3%) (10%), and with respect to ear infections the percentage of isolation that have emerged in this study are (3.33%) and isolated rate from the throat and sputum was (6.6%).

After a Diagnosis results have shown the prevalence of K.pneumoniae with rate(23 K.terrigena isolates 2with76.66%) followed by K.oxytoca rate (5with16.66%) and with( 6.66%) of the total 30 isolates.

Twenty isolates were selected belonging to the species K.pneumoniae and K.oxytoca which diagnosed by Api20E for rediagnosing by(PCR and viteke2) , and the results of the diagnostic system Vitek2 showd that the Klebsiella which have been previously diagnosed byApi20E system (90%) K.pneumoniae and (10%) K.oxytoca while the genetic diagnosis results showed 80% of the isolates belonged to the type of the K.pneumoniae and 20% of isolates belonging to K.oxytoca