The effect of Lactobacillus plantarum on expression of biofilm genes of Streptococcus mutans isolated from dental caries
Other Title(s)
تأثير بكتريا Lactobacillus plantarum على التعبير عن جينات الأغشية الحيوية للبكتريا Streptococcus mutans المعزولة من تسوس الأسنان
Joint Authors
al-Salam, Rasha Mundhir Uthman
Abbas, Basil Abd al-Zahrah
Said, Zahra K.
Source
The Medical Journal of Basrah University
Issue
Vol. 37, Issue 2 (31 Dec. 2019), pp.91-99, 9 p.
Publisher
University of Basrah College of Medicine
Publication Date
2019-12-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Topics
Abstract EN
Background: Dental caries and decay caused by Streptococcus mutans.
Sucrose, stimulates S.
mutans to yield insoluble glucans to form oral biofilm also called dental plaque to start process of caries.
The GtfB and LuxS genes of S.
mutans are responsible for development and evolution of biofilm.
Lactobacillus plantarum as probiotic can affect the biofilm formation of S.
mutans.
Aim of the study: To evaluate the effect of Lactobacillus plantarum against S.
mutans biofilm formation.
Results: Growing biofilm in the presence of sucrose was detected using 100 well microtiter plate crystal violet assay and biofilm formation by S.
mutans in the presence of Lactobacillus was detected.
Gene expression of biofilm forming genes (GtfB and LuxS) was quantified through Real- time PCR.
The transcriptional levels of GtfB and LuxS genes were remarkably down regulated 9.43 -and 4.24- fold change, respectively, when treated with lactobacillus plantarum in comparison with the control group.
Conclusions: Lactobacillus plantarum as probiotics can be used effectively to reduce the chance of dental caries by decreasing the effects of S.
mutans and their gene expression to conserve good oral health.
American Psychological Association (APA)
Said, Zahra K.& Abbas, Basil Abd al-Zahrah& al-Salam, Rasha Mundhir Uthman. 2019. The effect of Lactobacillus plantarum on expression of biofilm genes of Streptococcus mutans isolated from dental caries. The Medical Journal of Basrah University،Vol. 37, no. 2, pp.91-99.
https://search.emarefa.net/detail/BIM-946057
Modern Language Association (MLA)
Said, Zahra K.…[et al.]. The effect of Lactobacillus plantarum on expression of biofilm genes of Streptococcus mutans isolated from dental caries. The Medical Journal of Basrah University Vol. 37, no. 2 (2019), pp.91-99.
https://search.emarefa.net/detail/BIM-946057
American Medical Association (AMA)
Said, Zahra K.& Abbas, Basil Abd al-Zahrah& al-Salam, Rasha Mundhir Uthman. The effect of Lactobacillus plantarum on expression of biofilm genes of Streptococcus mutans isolated from dental caries. The Medical Journal of Basrah University. 2019. Vol. 37, no. 2, pp.91-99.
https://search.emarefa.net/detail/BIM-946057
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 98-99
Record ID
BIM-946057