The effect of Lactobacillus plantarum on expression of biofilm genes of Streptococcus mutans isolated from dental caries

Other Title(s)

تأثير بكتريا Lactobacillus plantarum على التعبير عن جينات الأغشية الحيوية للبكتريا Streptococcus mutans المعزولة من تسوس الأسنان

Joint Authors

al-Salam, Rasha Mundhir Uthman
Abbas, Basil Abd al-Zahrah
Said, Zahra K.

Source

The Medical Journal of Basrah University

Issue

Vol. 37, Issue 2 (31 Dec. 2019), pp.91-99, 9 p.

Publisher

University of Basrah College of Medicine

Publication Date

2019-12-31

Country of Publication

Iraq

No. of Pages

9

Main Subjects

Dental

Topics

Abstract EN

Background: Dental caries and decay caused by Streptococcus mutans.

Sucrose, stimulates S.

mutans to yield insoluble glucans to form oral biofilm also called dental plaque to start process of caries.

The GtfB and LuxS genes of S.

mutans are responsible for development and evolution of biofilm.

Lactobacillus plantarum as probiotic can affect the biofilm formation of S.

mutans.

Aim of the study: To evaluate the effect of Lactobacillus plantarum against S.

mutans biofilm formation.

Results: Growing biofilm in the presence of sucrose was detected using 100 well microtiter plate crystal violet assay and biofilm formation by S.

mutans in the presence of Lactobacillus was detected.

Gene expression of biofilm forming genes (GtfB and LuxS) was quantified through Real- time PCR.

The transcriptional levels of GtfB and LuxS genes were remarkably down regulated 9.43 -and 4.24- fold change, respectively, when treated with lactobacillus plantarum in comparison with the control group.

Conclusions: Lactobacillus plantarum as probiotics can be used effectively to reduce the chance of dental caries by decreasing the effects of S.

mutans and their gene expression to conserve good oral health.

American Psychological Association (APA)

Said, Zahra K.& Abbas, Basil Abd al-Zahrah& al-Salam, Rasha Mundhir Uthman. 2019. The effect of Lactobacillus plantarum on expression of biofilm genes of Streptococcus mutans isolated from dental caries. The Medical Journal of Basrah University،Vol. 37, no. 2, pp.91-99.
https://search.emarefa.net/detail/BIM-946057

Modern Language Association (MLA)

Said, Zahra K.…[et al.]. The effect of Lactobacillus plantarum on expression of biofilm genes of Streptococcus mutans isolated from dental caries. The Medical Journal of Basrah University Vol. 37, no. 2 (2019), pp.91-99.
https://search.emarefa.net/detail/BIM-946057

American Medical Association (AMA)

Said, Zahra K.& Abbas, Basil Abd al-Zahrah& al-Salam, Rasha Mundhir Uthman. The effect of Lactobacillus plantarum on expression of biofilm genes of Streptococcus mutans isolated from dental caries. The Medical Journal of Basrah University. 2019. Vol. 37, no. 2, pp.91-99.
https://search.emarefa.net/detail/BIM-946057

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 98-99

Record ID

BIM-946057