Embryo culture and in vitro clonal propagation of oak (Quercus aegilops L.)
Other Title(s)
زراعة الأجنة و الإكثار الدقيق خارج الجسم الحي للبلوط (Quercus aegilops L.)
Joint Authors
Fadl al-Din, Laylan Husayn
Tuma, Rafail Shlimon
Source
The Iraqi Journal of Agricultural Science
Issue
Vol. 51, Issue 1 (29 Feb. 2020), pp.347-355, 9 p.
Publisher
University of Baghdad College of Agriculture
Publication Date
2020-02-29
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Topics
Abstract EN
An attempt was done to develop a micropropagation protocol for oak using embryo culture.
Oak is considered a hard-to-root woody plant by conventional propagation methods, that’s why using tissue culture techniques is a very suitable alternative method.
For oak embryo culture, WPM was used and found to be better than MS medium for embryo germination which gave 66.13%.
As well as adding of GA3 to the medium improved the germination rate of embryos (43.25% and 82.25 %).
At initiation stage, WPM was used and found to be the best medium by giving the highest number of shoots/ explant which was 1.80, the highest number of leaves (15.17 leaves/ explant) and the longest shoots (1.42 cm) followed by MS medium then GD which gave the lowest parameters which gave 0.98 shoots/ explant, 7.20 leaves/ explant and 1.06 cm shoot length.
At shoot multiplication stage, BA was better than Kinetin for multiplication of oak explants.
The addition of BA at 3 mg.l-l gave the highest number of shoot and leaves which were 3.33 and 26.11 respectively.
The longest shoots were achieved when 4.5 mg.l-l of BA was used.
Furthermore, kinetin at 3 and 4.5 mg-l gave the lowest parameters which were 1 cm in length and 1.54 leaves/ explant.
For rooting stage, NAA was better than IAA in giving better parameters and rooting percentage.
The highest number of roots and rooting percentage were achieved when 1 mg.l-l was added by giving 6 roots/ explant and 100% rooting percentage.
While the longest roots were achieved when 0.5 mg.l-l of NAA was used (3.67 cm) followed by 1.5 mg.l-l IAA which gave 3.55 roots/ explant with rooting percentage 90%.
The produced plantlets were successfully acclimatized and transferred to the open-air conditions with a rate reached 85%.
American Psychological Association (APA)
Fadl al-Din, Laylan Husayn& Tuma, Rafail Shlimon. 2020. Embryo culture and in vitro clonal propagation of oak (Quercus aegilops L.). The Iraqi Journal of Agricultural Science،Vol. 51, no. 1, pp.347-355.
https://search.emarefa.net/detail/BIM-948710
Modern Language Association (MLA)
Fadl al-Din, Laylan Husayn& Tuma, Rafail Shlimon. Embryo culture and in vitro clonal propagation of oak (Quercus aegilops L.). The Iraqi Journal of Agricultural Science Vol. 51, no. 1 (2020), pp.347-355.
https://search.emarefa.net/detail/BIM-948710
American Medical Association (AMA)
Fadl al-Din, Laylan Husayn& Tuma, Rafail Shlimon. Embryo culture and in vitro clonal propagation of oak (Quercus aegilops L.). The Iraqi Journal of Agricultural Science. 2020. Vol. 51, no. 1, pp.347-355.
https://search.emarefa.net/detail/BIM-948710
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 354-355
Record ID
BIM-948710