Diagnosis of reovirus infection in broiler breeders flocks by using PCR technique in Erbil province
Other Title(s)
تشخيص الإصابة بفيروس الريو في أمهات فروج اللحم باستخدام تقنية تفاعل البلمرة المتسلسل في محافظة أربيل
Author
Source
Iraqi Journal of Veterinary Sciences
Issue
Vol. 34, Issue 1 (30 Jun. 2020), pp.77-81, 5 p.
Publisher
University of Mosul College of Veterinary Medicine
Publication Date
2020-06-30
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Abstract EN
Avian reoviruses can infect birds without any clinical signs of infection, the infection may associate with different manifestations including viral arthritis/tenosynovitis and malabsorption syndrome.
The objective of this study was to use advance methods representing by molecular methods (RT-PCR, RT-qPCR) in the diagnosis of ARV infection in broiler breeders' flocks.
A 4 flocks of broiler breeders (ROSS breed) 39 weeks age with approximately 10% morbidity rate due to Avian Reovirus (ARV).
The clinical examination of 16 infected birds revealed unilateral lameness and swelling of hock joint.
Blood samples were collected from wing vein of infected birds.
Sera were tested for antibodies titer against ARV and Mycoplasma synoviae (MS).
5 of 16 positive samples were selected randomly for amplification by RT-PCR and RT-qPCR.
The results showed in postmortem examination of infected birds, unilateral arthritis with visible joint lesions.
Antibodies titer measured by ELISA in the sera of birds after 4 and 20 weeks of infection with ARV was positive and high.
In RT- PCR 1 of 5 samples gave positive reaction for amplification while in RT-qPCR all five samples gave positive results for amplification in comparison with +ve and -ve control.
American Psychological Association (APA)
Danial, Fanar Ablahad Ishaq. 2020. Diagnosis of reovirus infection in broiler breeders flocks by using PCR technique in Erbil province. Iraqi Journal of Veterinary Sciences،Vol. 34, no. 1, pp.77-81.
https://search.emarefa.net/detail/BIM-948790
Modern Language Association (MLA)
Danial, Fanar Ablahad Ishaq. Diagnosis of reovirus infection in broiler breeders flocks by using PCR technique in Erbil province. Iraqi Journal of Veterinary Sciences Vol. 34, no. 1 (2020), pp.77-81.
https://search.emarefa.net/detail/BIM-948790
American Medical Association (AMA)
Danial, Fanar Ablahad Ishaq. Diagnosis of reovirus infection in broiler breeders flocks by using PCR technique in Erbil province. Iraqi Journal of Veterinary Sciences. 2020. Vol. 34, no. 1, pp.77-81.
https://search.emarefa.net/detail/BIM-948790
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 81
Record ID
BIM-948790