Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq

Other Title(s)

دراسة التنميط الجيني للمتورقة العملاقة المعزولة من الماشية في مدينة عقرة، العراق

Joint Authors

Mustafa, Nashat Ghalib
Hammu, Rada Nazim
al-Rabii, Fuad Ismail

Source

Iraqi Journal of Veterinary Sciences

Issue

Vol. 34, Issue 1 (30 Jun. 2020), pp.123-127, 5 p.

Publisher

University of Mosul College of Veterinary Medicine

Publication Date

2020-06-30

Country of Publication

Iraq

No. of Pages

5

Main Subjects

Zoology

Topics

Abstract EN

This study was conducted to investigate the 18S rRNA gene of Fasciola gigantica obtained from the liver of cattle live in Aqrah city, Iraq.

Fifty-nine Fasciola flukes were collected through routine investigation from livers of naturally infected local cattle (42 cows), from May to August 2017, at the central slaughterhouse of Aqrah city, Kurdistan region of Iraq, the flukes were washed by PBS and then fixed in ethanol.

Genomic DNA was extracted, and a 560 bp fragment was amplified by PCR, subsequent by sequencing of PCR products.

A remarkable result of this project was the deposition of our gene isolate in GenBank (Accession No.

MG786553).

However, it was confirmed by the sequence results that isolate species was F.

gigantica, and interestingly our samples sequences have alignment match of 100% with many international isolates, without genetic mutations or variations.

It is concluded that molecular study could be utilized for both diagnosis and differential diagnosis of parasites with huge precise.

Also, an 18S rRNA gene is a perfect fragment for molecular study and phylogenetic analysis of F.

gigantica, also our samples have 100% alignment match with universal isolates.

American Psychological Association (APA)

Hammu, Rada Nazim& al-Rabii, Fuad Ismail& Mustafa, Nashat Ghalib. 2020. Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq. Iraqi Journal of Veterinary Sciences،Vol. 34, no. 1, pp.123-127.
https://search.emarefa.net/detail/BIM-948819

Modern Language Association (MLA)

Hammu, Rada Nazim…[et al.]. Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq. Iraqi Journal of Veterinary Sciences Vol. 34, no. 1 (2020), pp.123-127.
https://search.emarefa.net/detail/BIM-948819

American Medical Association (AMA)

Hammu, Rada Nazim& al-Rabii, Fuad Ismail& Mustafa, Nashat Ghalib. Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq. Iraqi Journal of Veterinary Sciences. 2020. Vol. 34, no. 1, pp.123-127.
https://search.emarefa.net/detail/BIM-948819

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 126-127

Record ID

BIM-948819