Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq
Other Title(s)
دراسة التنميط الجيني للمتورقة العملاقة المعزولة من الماشية في مدينة عقرة، العراق
Joint Authors
Mustafa, Nashat Ghalib
Hammu, Rada Nazim
al-Rabii, Fuad Ismail
Source
Iraqi Journal of Veterinary Sciences
Issue
Vol. 34, Issue 1 (30 Jun. 2020), pp.123-127, 5 p.
Publisher
University of Mosul College of Veterinary Medicine
Publication Date
2020-06-30
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Topics
Abstract EN
This study was conducted to investigate the 18S rRNA gene of Fasciola gigantica obtained from the liver of cattle live in Aqrah city, Iraq.
Fifty-nine Fasciola flukes were collected through routine investigation from livers of naturally infected local cattle (42 cows), from May to August 2017, at the central slaughterhouse of Aqrah city, Kurdistan region of Iraq, the flukes were washed by PBS and then fixed in ethanol.
Genomic DNA was extracted, and a 560 bp fragment was amplified by PCR, subsequent by sequencing of PCR products.
A remarkable result of this project was the deposition of our gene isolate in GenBank (Accession No.
MG786553).
However, it was confirmed by the sequence results that isolate species was F.
gigantica, and interestingly our samples sequences have alignment match of 100% with many international isolates, without genetic mutations or variations.
It is concluded that molecular study could be utilized for both diagnosis and differential diagnosis of parasites with huge precise.
Also, an 18S rRNA gene is a perfect fragment for molecular study and phylogenetic analysis of F.
gigantica, also our samples have 100% alignment match with universal isolates.
American Psychological Association (APA)
Hammu, Rada Nazim& al-Rabii, Fuad Ismail& Mustafa, Nashat Ghalib. 2020. Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq. Iraqi Journal of Veterinary Sciences،Vol. 34, no. 1, pp.123-127.
https://search.emarefa.net/detail/BIM-948819
Modern Language Association (MLA)
Hammu, Rada Nazim…[et al.]. Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq. Iraqi Journal of Veterinary Sciences Vol. 34, no. 1 (2020), pp.123-127.
https://search.emarefa.net/detail/BIM-948819
American Medical Association (AMA)
Hammu, Rada Nazim& al-Rabii, Fuad Ismail& Mustafa, Nashat Ghalib. Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq. Iraqi Journal of Veterinary Sciences. 2020. Vol. 34, no. 1, pp.123-127.
https://search.emarefa.net/detail/BIM-948819
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 126-127
Record ID
BIM-948819