تحديد وسط الزرع الملائم و تأثير مستخلص الحناء Lawsonia inermis L. في وسط الزراعة النسيجية على إنتاج نبيتات خالية من فيروس البطاطا-البطاطس Y

Abstract EN

This study was conducted to determine the more favorable medium of tissue culture to induce direct organogenesis from leaf and stem parts of potato plant and evaluate the effect of Lawsonia inermis L.

(Henna) extract in the medium in obtaining plantlets free of Potato virus Y (PVY).

Results showed that the Murashige and Skooge (MS) medium amended with 4.52 mg/l benzyladenine (BA) and 10 mg/l Gibberellic acid (GA3) was the most effective medium in inducing direct organogenesis from leaf and stem explants of potato (Desiree cultivar), and the formation of virus free shoots from infected parts.

The addition of Henna extract to the culture medium was found to be very effective in the elimination of PVY.

Hundred percent of virus free plantlets was obtained when 50 mg/l of Henna extract were added to the solid medium culture.

Percentage of 0, 33.3, 46.2, 58.3, 60.0, 91.7 and 100 of plantlets free of virus were obtained when 0, 50, 100, 200, 300, 400, and 500 mg/l were added to the liquid media.

The highest concentration (500 mg/l) caused a delay of plantlets growth