Development and validation of RP-HPLC method for quantification of fluconazole in pharmaceutical formulations

Other Title(s)

التطوير و التحقق من صحة طريقة الفصل الكروماتوجرافي السائل عالي الأداء الطور العكسي لتقدير كمية الفلوكونازول في المستحضرات الصيدلانية

Joint Authors

Filimban, Ala Muhammad Jamil
Abu al-Ula, Amal al-Sayih Fadl
al-Anzi, Fadilah Sfuq
Taha, Ihab

Source

Journal of Medical and Pharmaceutical Sciences

Issue

Vol. 4, Issue 1 (31 Mar. 2020), pp.27-35, 9 p.

Publisher

National Research Center

Publication Date

2020-03-31

Country of Publication

Palestine (Gaza Strip)

No. of Pages

9

Main Topic

Pharmacology

Topics

Abstract EN

A rapid and simple reversed phase high performance liquid chromatography method to quantify fluconazole which is a triazole antifungal drug that is available in pharmaceutical oral dosage forms (tablets and suspension) and parenteral intravenous injection.

It is used mainly against Candida albicans.

The chromatography was carried out using Nova-Pak C18 (3.9 x 150 mm) Column at 260 nm wave length using a photodiode array detector at room temperature.

The mobile phase consisted of 0.01 M phosphate buffer of pH 7 and acetonitrile in a ratio of 75:25 v/v delivered at a flow rate of 1 ml/min.

The retention time was 2 min with a short run time of 2.5 min.

The method was validated with respect to linearity, precision, accuracy, and specificity.

The linearity was established over the concentration range between 1-200 μg/ml.

The percentage of drug recovered after analysis for Intra-day was between 92.64% and 108.00% while the inter-day was between 93.17% and 100.25%.

The proposed method is easy to apply, it is a fast method with a retention time of only two minutes making it perfect for determining routine analysis with low fluconazole sample concentrations and it is the quickest method ever recorded for fluconazole analysis by HPLC.

American Psychological Association (APA)

Filimban, Ala Muhammad Jamil& Abu al-Ula, Amal al-Sayih Fadl& al-Anzi, Fadilah Sfuq& Taha, Ihab. 2020. Development and validation of RP-HPLC method for quantification of fluconazole in pharmaceutical formulations. Journal of Medical and Pharmaceutical Sciences،Vol. 4, no. 1, pp.27-35.
https://search.emarefa.net/detail/BIM-969626

Modern Language Association (MLA)

Filimban, Ala Muhammad Jamil…[et al.]. Development and validation of RP-HPLC method for quantification of fluconazole in pharmaceutical formulations. Journal of Medical and Pharmaceutical Sciences Vol. 4, no. 1 (Mar. 2020), pp.27-35.
https://search.emarefa.net/detail/BIM-969626

American Medical Association (AMA)

Filimban, Ala Muhammad Jamil& Abu al-Ula, Amal al-Sayih Fadl& al-Anzi, Fadilah Sfuq& Taha, Ihab. Development and validation of RP-HPLC method for quantification of fluconazole in pharmaceutical formulations. Journal of Medical and Pharmaceutical Sciences. 2020. Vol. 4, no. 1, pp.27-35.
https://search.emarefa.net/detail/BIM-969626

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p.34-35

Record ID

BIM-969626