Molecular detection of some Anaplasma Species in blood of dogs in Baghdad province, Iraq

Other Title(s)

الكشف الجزيئي لبعض أنواع جراثيم Anaplasma في محافظه بغداد-العراق

Joint Authors

al-Ani, Jinan Mahmud Khalaf
al-Ubaydi, Suha Shihab Ahmad
al-Shammari, Nusayr Badawi

Source

Iraqi Journal of Veterinary Medicine

Issue

Vol. 44, Issue 1 (30 Jun. 2020), pp.39-45, 7 p.

Publisher

University of Baghdad College of Veterinary Medicine

Publication Date

2020-06-30

Country of Publication

Iraq

No. of Pages

7

Main Topic

Veterinary Medicine

Topics

Abstract AR

من مجموع 150 عينه دم تم جمعها من الكلاب في محافظة بغداد فحصت عينات الدم بصبغها بصبغه الكيمزا و اجرى تفاعل سلسلة خمير ةالبلمرة باستخدام بوادئ Primer خاص بجين ) 16S rRNA ( للمسبب المرضي و بعدها تسلسل القواعد النيتروجينية لبعض العينات المصابة.

تم التشخيص التاكيدي بواسطه الحمض النووي في الدم و كان معدل الاصاب ة الكلي 12 / 150 ( 8%(، ظهرت النتائج 5 عينه من 12 عينه دممصابة بال A.

platys) 41.

67% ) و بلغ اجمالي الكلاب المصابة بال A.

phagocytophilum مع معدل اصابه 7 / 12 (.

58.

33.

)% تم اجراءالفحص التاكيدي بواسطه تسلسل القواعد النيتروجينية باستخدام خمسه عشر مراجع من الجينات 16S rRNA من A.

platys و اربعه عشرمراجع من الجينات 16S rRNA من A.

phagocytophilum المسجلة في بنك الجينات، تراوحت درجة التطابق و التشابه بين النتائج فيعزلاتنا مع السلالات المرجعية من 98 % الى 99.

%

Abstract EN

A total of 150 blood samples were collected from dogs and examined by the polymerase chain reaction (PCR) technique, which was used to detect the 16S RNA gene of Anaplasma platys and Anaplasma phagocytophilum.

Subsequent analysis of the PCR amplicons was achieved by nucleotides sequencing of some positive samples.

Totally, the findings show the presence of PCR products (i.

e.

Anaplasma spp.

infection) in 12/150 (8.

0%) of the dogs under study.

While 5/150 (3.

33%) of the cases were A.

platys, 7/150 (4.

66%) were A.

phagocytophilum.

Nucleotide sequencing confirmed the identity of the amplified genes whose sequences were compared with other references belong to 15 of 16S rRNA gene of A platys and 14 references of 16S rRNA gene of A.

phagocytophilum, and the sequences of this study isolates were deposited on the Gene Bank.

The identity and similarity scores between the isolates of this study and reference strains ranged from 98 to 99%.

In conclusion, canine anaplasmosis prevalence in dogs could be underestimated in Iraq, and the phylogenetic tree of the local A.

platys and A.

phagocytophilum isolates were found to resemble other worldwide strains of Anaplasma spp.

with a high degree of similarity.

American Psychological Association (APA)

al-Ubaydi, Suha Shihab Ahmad& al-Ani, Jinan Mahmud Khalaf& al-Shammari, Nusayr Badawi. 2020. Molecular detection of some Anaplasma Species in blood of dogs in Baghdad province, Iraq. Iraqi Journal of Veterinary Medicine،Vol. 44, no. 1, pp.39-45.
https://search.emarefa.net/detail/BIM-973009

Modern Language Association (MLA)

al-Ubaydi, Suha Shihab Ahmad…[et al.]. Molecular detection of some Anaplasma Species in blood of dogs in Baghdad province, Iraq. Iraqi Journal of Veterinary Medicine Vol. 44, no. 1 (2020), pp.39-45.
https://search.emarefa.net/detail/BIM-973009

American Medical Association (AMA)

al-Ubaydi, Suha Shihab Ahmad& al-Ani, Jinan Mahmud Khalaf& al-Shammari, Nusayr Badawi. Molecular detection of some Anaplasma Species in blood of dogs in Baghdad province, Iraq. Iraqi Journal of Veterinary Medicine. 2020. Vol. 44, no. 1, pp.39-45.
https://search.emarefa.net/detail/BIM-973009

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 43-44

Record ID

BIM-973009