Nanoparticle-membrane interactions : a systematic study of the electric charge contribution to the wrapping process

Other Title(s)

تفاعل الجسيمات النانوية مع جدار الخلية : دراسة منهجية لدور الشحنة الكهربائية لهذه الجسيمات في عملية دخولها

Dissertant

Uthman, Samih M.

Thesis advisor

Sayyid Ahmad, Abd Allah
Auth, Thorsten

University

Birzeit University

Faculty

Faculty of Science

Department

Department of Physics

University Country

Palestine (West Bank)

Degree

Master

Degree Date

2020

English Abstract

In this study, we are interested in developing a physically motivated model to predict the affinity of a nanoparticle to be wrapped by a cell membrane.

This model is potentially useful for medical diagnostics, therapy and basic research.

Traditionally, nanoparticle wrapping states are characterized by energies based on nanoparticlemembrane adhesion-energy gain and membrane deformation-energy cost.

However, to calculate the interactions between nanoparticles and biological cells it is important to take into account the multi-component nature of the cell membranes.

Furthermore, although often strongly screened, electrostatic interactions are essential as they affect the behaviour of a nanoparticle-membrane system.

For example, the presence of a charged particle next to a multi-component lipid-bilayer membrane leads to the accumulation of oppositely charged proteins and lipids around it.

Our model consists of a spherical nanoparticle with a surface charge and specific dielectric properties wrapped by a membrane.

The membrane shape for a given wrapping state is obtained from minimizing the Helfrich Hamiltonian.

The membrane is represented by a lipid bilayer.

It has a hydrophobic core and possibly charged hydrophilic surfaces with mobile charges impeded in them.

Additionally, both the core and surface regions are assigned distinct dielectric properties.

Lastly, the intracellular and extracellular media are represented by electrolyte solutions with different salt concentrations.

We solve the axisymmetric nonlinear Poisson-Boltzmann equation to calculate electrostatic free energy.

Our results indicate that electrical charges can significantly alter the interaction of spherical nanoparticles with lipid bilayers.

They also suggest that the interplay among electrostatic interactions, charge redistribution, wrapping fraction of the nanoparticle and lipid-bilayer deformation govern the wrapping process.

Main Subjects

Physics

Topics

No. of Pages

55

Table of Contents

Table of contents.

Abstract.

Abstract in Arabic.

Chapter One : Introduction.

Chapter Two : Methods.

Chapter Three : Results.

Chapter Four : Conclusions.

References.

American Psychological Association (APA)

Uthman, Samih M.. (2020). Nanoparticle-membrane interactions : a systematic study of the electric charge contribution to the wrapping process. (Master's theses Theses and Dissertations Master). Birzeit University, Palestine (West Bank)
https://search.emarefa.net/detail/BIM-977585

Modern Language Association (MLA)

Uthman, Samih M.. Nanoparticle-membrane interactions : a systematic study of the electric charge contribution to the wrapping process. (Master's theses Theses and Dissertations Master). Birzeit University. (2020).
https://search.emarefa.net/detail/BIM-977585

American Medical Association (AMA)

Uthman, Samih M.. (2020). Nanoparticle-membrane interactions : a systematic study of the electric charge contribution to the wrapping process. (Master's theses Theses and Dissertations Master). Birzeit University, Palestine (West Bank)
https://search.emarefa.net/detail/BIM-977585

Language

English

Data Type

Arab Theses

Record ID

BIM-977585