Polymorphic of DGAT1 gene (Intron 1)‎ and their relationship to performance in Iraqi buffalo

Abstract EN

This study was conducted in two locations (al-Fadelian and Althahab al'abaith) in Baghdad governorate on a sample of 60 Iraqi buffalo females (Bubalus bubalis) for the period from 1/11/2017 to 1/11/2018.

This study was conducted to: identifying the genotypes, the proportions of their distribution of fragment (Intron1-1146 bp) of DGAT1 gene and the relationship of those genotypes to performance of the Buffalo were the main goals of this study.

The genotypes of the target non-coding area of the specific Fragment (Intron1-1146 bp) differed from DGAT1 gene depending on the different genetic packets resulting from enzymatic digestion, which showed three genotypes represented by CC, CT and TT and their distribution ratios were 60.00, 33.33 and 6.67% Respectively, the discrepancy between these three ratios was highly significant (P < 0.01), while the frequency of allele was 0.77 and 0.23 for both allele's C and T respectively.

The results showed that the production of daily milk was not significantly affected by the different in the genotypes of this Fragment of DGAT1 gene, while the age of the first birth was significant affected (P < 0.05) by the variation of the genotypes for the studied fragment by DGAT1 gene of buffalo with genotype CT and CC in comparison with its peers that had the genotype TT.

The fat ratio had been significantly influenced where it had been at maximum in buffalo milk that had the genotype TT (8.00 ± 0.52 % ) and CT (7.43 ± 0.71 % ), while other milk component ratio had not been significantly affected by the genotype variation.

The body dimensions also had not been significantly affected at mother except chest circumference (P<0.05), the highest production was in buffalo with TT genotype (213.00± 0.00cm).