Sero-diagnosis of bovine tuberculosis using a rapid lateral flow test and elisa in dairy cattle in Egypt

Abstract EN

Mycobacterium bovis (M.

bovis), the cause of bovine tuberculosis (BTB) in cattle, remains a major zoonotic and economic problem in many countries.

This study aimed to apply different serological tests for detection of bovine tuberculosis as a complementary for intradermal tuberculin test.

The tested 4250 cattle in different Egyptian governorates by single intradermal cervical comparative tuberculin test (SICCT), 90 (2.1 %) cattle reacted positively.

The postmortem (PM) examination of the positive reactors revealed 65 (72.2 %) out of 90 slaughtered cattle with visible lesions (VL) while the other 25 (27.8 %) reactors showed no visible lesions (NVL).

The visible lesions detected in head were 10 (15.4 %) out of the 65 VL, in respiratory system were 32 (49.2%), in the digestive system were 5 (7.7 %), in mixed infection were 11 (16.9 %), and in generalized infection were 7(10.8 %).

The bacteriological examination of processed samples from the 90 slaughtered cattle revealed 55 M.

bovis (61.1%); 51 (78.5 %) were from 65 VL and 4 (16.0%) were from 25 NVL.

Enzyme linked immune sorbent assay (ELISA) was used for the tuberculin positive animals’ sera and gave 36 (55.4%) out of 65 cattle with and 2 (8 %) out of 25 cattle with NVL by a total 38 (42.2 %) out of the 90 tuberculin positive animals.

The results of lateral flow test gave 32 (49.2 %) out of 65 cattle with VL and 1 (4 %) out of 25 cattle with NVL by a total 33 (36.7 %) out of the 90 tuberculin positive animals.